Dosik G M, Barlogie B, Göhde W, Johnston D, Tekell J L, Drewinko B
Blood. 1980 May;55(5):734-40.
Because cytokinetic studies of the human bone marrow aspirate as a prognostic factor and as a monitor of drug perturbation are frequently inconsistent, we investigated reproducibility of DNA distribution measured by flow cytometry of DNA content in patients with morphologically normal bone marrow. In 15 patients, correlation was noted between DNA distributions simultaneously obtained on right and left iliac crest bone marrow aspirates (r = .588), although considerable variation in individuals was encountered. Much better reproducibility (r = .879) was achieved using bilateral core biopsy of bone marrow in these same patients. In 60 samples, comparison of DNA distribution between bone marrow aspirate and simultaneously obtained biopsy revealed higher relative proportions of S and G2 + M phase cells in biopsies (p less than 0.001), suggesting peripheral blood contamination of aspirate material. Brisk shaking of biopsy specimens in saline expelled a representative sample in the supernatant that could be subjected to simultaneous cytomorphological and cytokinetic analysis. To improve reproducibility of DNA content determinations in normal human bone marrow, bone marrow biopsy should be utilized.
由于将人类骨髓穿刺物的细胞动力学研究作为预后因素以及药物干扰监测指标时，结果常常不一致，我们对形态学正常的骨髓患者通过流式细胞术测量DNA含量来研究DNA分布的可重复性。在15名患者中，发现左右髂嵴骨髓穿刺物同时获得的DNA分布之间存在相关性（r = 0.588），尽管个体间存在相当大的差异。在这些相同患者中，使用双侧骨髓芯针活检可获得更好的可重复性（r = 0.879）。在60个样本中，骨髓穿刺物与同时获得的活检组织之间的DNA分布比较显示，活检组织中S期和G2 + M期细胞的相对比例更高（p < 0.001），提示穿刺物材料受到外周血污染。在盐水中剧烈摇晃活检标本可使上清液中排出一个代表性样本，该样本可同时进行细胞形态学和细胞动力学分析。为提高正常人骨髓DNA含量测定的可重复性，应采用骨髓活检。
