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[粪便中轮状病毒的快速检测。乳胶试验的价值]

[Rapid detection of rotaviruses in feces. Value of a latex test].

作者信息

Agius G, Baillargeau E, Castets M, Samb A

出版信息

Pathol Biol (Paris). 1984 Jan;32(1):56-8.

PMID:6701008
Abstract

We have sought the presence of rotaviruses in 114 fecal samples. Among them, 14 were from African children and the remainder were samples sent to the Poitiers Hospital Virology Laboratory. Three techniques were applied to each sample: latex agglutination (LTX), electron microscopy (EM) after negative staining and ELISA (Enzyme Linked Immunosorbent Assay). 64% of the samples were negative with the three methods. 19.3% were positive with EM and ELISA. The LTX test showed 16.7% positives among these 19.3%. Our series furnished 5.2% uninterpretable results and 3.5% positive uniquely with the LTX test. ELISA is the most sensitive and the most specific technique but LTX is useful for mass screenings. The advantages of LTX include rapidity, simplicity, reduced instrumentation and low cost price. We suggest the possibility of large scale use of this test in countries with a relatively undeveloped hygienic infrastructure where malnutrition of children aggravates rotavirus infections.

摘要

我们对114份粪便样本进行了轮状病毒检测。其中14份来自非洲儿童,其余样本则是送往普瓦捷医院病毒学实验室的样本。对每份样本都采用了三种检测技术:乳胶凝集试验(LTX)、负染后电子显微镜检查(EM)以及酶联免疫吸附测定(ELISA)。64%的样本在这三种方法检测下均呈阴性。19.3%的样本经EM和ELISA检测呈阳性。在这19.3%呈阳性的样本中,LTX检测显示阳性率为16.7%。我们的样本系列中有5.2%的结果无法解读,还有3.5%的样本仅通过LTX检测呈阳性。ELISA是最灵敏且最具特异性的技术,但LTX对于大规模筛查很有用。LTX的优点包括检测速度快、操作简单、所需仪器设备少且成本低廉。我们建议,在卫生基础设施相对不发达、儿童营养不良会加重轮状病毒感染的国家,有可能大规模使用这项检测。

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