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1
[Detection of human rotaviruses in faeces : comparison of five methods].[粪便中人类轮状病毒的检测:五种方法的比较]
Med Mal Infect. 1985 Dec;15(12):737-740. doi: 10.1016/S0399-077X(85)80323-1. Epub 2005 Jun 20.
2
Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests.通过比较两种酶免疫测定法和两种乳胶试验,对乳胶凝集试验进行直接评估,乳胶凝集试验是检测儿童肠胃炎中轮状病毒的一种便捷的酶免疫测定替代方法。
J Clin Microbiol. 1985 Apr;21(4):622-5. doi: 10.1128/jcm.21.4.622-625.1985.
3
[Usefulness of immunoenzyme diagnostic kits and latex tests for isolation of rotaviruses].[免疫酶诊断试剂盒和乳胶试验在轮状病毒分离中的应用价值]
Med Dosw Mikrobiol. 1990;42(1-2):54-60.
4
Evaluation of two commercial kits for rapid detection of human rotavirus in feces: Rotalex, a latex agglutination test and Rotavirus ELISA Kit.两种用于粪便中人类轮状病毒快速检测的商业试剂盒评估:乳胶凝集试验Rotalex和轮状病毒酶联免疫吸附测定试剂盒。
Acta Pathol Microbiol Immunol Scand B. 1985 Apr;93(2):159-60. doi: 10.1111/j.1699-0463.1985.tb02869.x.
5
[Infectious viral diarrheas. Comparison of research methods for rotaviruses].[传染性病毒性腹泻。轮状病毒研究方法的比较]
Ann Biol Clin (Paris). 1986;44(4):406-9.
6
Detection of rotavirus by a latex agglutination test, Rotalex: comparison with electron microscopy, immunofluorescence, polyacrylamide gel electrophoresis, and enzyme linked immunosorbent assay.用乳胶凝集试验(Rotalex)检测轮状病毒:与电子显微镜检查、免疫荧光法、聚丙烯酰胺凝胶电泳及酶联免疫吸附测定法的比较
J Clin Pathol. 1985 Jun;38(6):694-700. doi: 10.1136/jcp.38.6.694.
7
Comparison of indirect double antibody and double antibody sandwich ELISA techniques with latex agglutination test for the diagnosis of human rotavirus infection.间接双抗体和双抗体夹心ELISA技术与乳胶凝集试验在诊断人轮状病毒感染中的比较。
Acta Virol. 1984 Jan;28(1):59-63.
8
Comparison of six methods for detecting human rotavirus in stools.六种检测粪便中人类轮状病毒方法的比较。
Eur J Clin Microbiol. 1984 Apr;3(2):136-40. doi: 10.1007/BF02014331.
9
Multicentre evaluation of a new commercial latex agglutination test using a monoclonal antibody for rotavirus detection.使用单克隆抗体进行轮状病毒检测的新型商用乳胶凝集试验的多中心评估。
Eur J Clin Microbiol Infect Dis. 1989 Mar;8(3):251-3. doi: 10.1007/BF01965271.
10
Evaluation of a new latex agglutination test for detecting human rotavirus in faeces.一种用于检测粪便中人类轮状病毒的新型乳胶凝集试验的评估。
J Infect. 1983 Sep;7(2):130-3. doi: 10.1016/s0163-4453(83)90527-3.

引用本文的文献

1
Detection of rotavirus in faecal specimens with a monoclonal antibody enzyme-linked immunosorbent assay: comparison with polyclonal antibody enzyme immuno-assays and a latex agglutination test.用单克隆抗体酶联免疫吸附测定法检测粪便标本中的轮状病毒:与多克隆抗体酶免疫测定法和乳胶凝集试验的比较
Comp Immunol Microbiol Infect Dis. 1989;12(4):95-104. doi: 10.1016/0147-9571(89)90058-1.

本文引用的文献

1
[Rapid detection of rotaviruses in feces. Value of a latex test].[粪便中轮状病毒的快速检测。乳胶试验的价值]
Pathol Biol (Paris). 1984 Jan;32(1):56-8.
2
Comparison of six methods for detecting human rotavirus in stools.六种检测粪便中人类轮状病毒方法的比较。
Eur J Clin Microbiol. 1984 Apr;3(2):136-40. doi: 10.1007/BF02014331.
3
Rapid detection of rotavirus in stool by latex agglutination: comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test.通过乳胶凝集法快速检测粪便中的轮状病毒:与放射免疫测定法和电子显微镜检查的比较及该检测方法的临床评估
J Med Virol. 1983;11(2):91-7. doi: 10.1002/jmv.1890110202.
4
Detection of a rotavirus-like agent associated with diarrhea in an infant.在一名婴儿中检测到与腹泻相关的轮状病毒样病原体。
J Clin Microbiol. 1982 Oct;16(4):724-6. doi: 10.1128/jcm.16.4.724-726.1982.
5
Solid-phase enzyme immunoassay for rotavirus antigen: faecal protease activity as a reason for false-negative results.轮状病毒抗原的固相酶免疫测定:粪便蛋白酶活性导致假阴性结果的原因
J Virol Methods. 1982 Sep;5(1):45-53. doi: 10.1016/0166-0934(82)90096-9.
6
Comparison of direct and indirect enzyme immunoassays with direct ultracentrifugation before electron microscopy for detection of rotaviruses.在电子显微镜检查前,直接和间接酶免疫测定法与直接超速离心法检测轮状病毒的比较。
J Clin Microbiol. 1982 Jul;16(1):53-9. doi: 10.1128/jcm.16.1.53-59.1982.
7
Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus.酶免疫测定法与电子显微镜检测程序在轮状病毒检测中的比较。
J Clin Microbiol. 1982 May;15(5):938-44. doi: 10.1128/jcm.15.5.938-944.1982.
8
Evaluation of enzyme immunoassays for the detection of human rotavirus.用于检测人轮状病毒的酶免疫测定法的评估
J Infect Dis. 1981 Oct;144(4):379. doi: 10.1093/infdis/144.4.379.
9
Detection of rotavirus in faeces by latex agglutination.通过乳胶凝集试验检测粪便中的轮状病毒。
J Immunol Methods. 1981;41(3):377-85. doi: 10.1016/0022-1759(81)90199-x.
10
[Evaluation of a new latex test for detecting human rotaviruses in feces].[一种用于检测粪便中人类轮状病毒的新型乳胶试验的评估]
Pathol Biol (Paris). 1985 Jun;33(6):681-3.

[粪便中人类轮状病毒的检测:五种方法的比较]

[Detection of human rotaviruses in faeces : comparison of five methods].

作者信息

Baillargeau E, Ranger S, Agius G, Samb A, Castets M, Rekas B

机构信息

Laboratoire de Microbiologie B, Centre Hospitalier Universitaire, BP 577, F 86021 Poitiers Cédex France.

Laboratoire de Bactériologie-Virologie, Centre Hospitalier Universitaire, Dakar, Sénégal.

出版信息

Med Mal Infect. 1985 Dec;15(12):737-740. doi: 10.1016/S0399-077X(85)80323-1. Epub 2005 Jun 20.

DOI:10.1016/S0399-077X(85)80323-1
PMID:38620261
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7131474/
Abstract

The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specificity for all techniques were satisfactory. Comparison of the methods showed Enzygnost to be the most sensitive (89 %), followed by the two agglutination tests, then electron microscopy and least sensitive the Bio-Enza-Bead (56 %). For large - scale screening of faeces, it appeared that the latex agglutination test were preferable for reasons of simplicity, speed and cost.

摘要

作者采用五种用于检测人类粪便中轮状病毒的技术,对118份粪便样本进行了检测,这五种技术分别是:电子显微镜检查、乳胶凝集试验(Slidex Rota-kit:生物梅里埃公司;Rotalex:奥立安诊断公司)和酶联免疫吸附测定(Enzygnost:贝林公司;Bio-Enza-Bead:Bionetics公司)。所有技术的重现性和特异性均令人满意。方法比较显示,Enzygnost最为灵敏(89%),其次是两种凝集试验,然后是电子显微镜检查,最不灵敏的是Bio-Enza-Bead(56%)。对于粪便的大规模筛查,出于操作简便、速度快和成本低的原因,乳胶凝集试验似乎更为可取。