Baillargeau E, Ranger S, Agius G, Samb A, Castets M, Rekas B
Laboratoire de Microbiologie B, Centre Hospitalier Universitaire, BP 577, F 86021 Poitiers Cédex France.
Laboratoire de Bactériologie-Virologie, Centre Hospitalier Universitaire, Dakar, Sénégal.
Med Mal Infect. 1985 Dec;15(12):737-740. doi: 10.1016/S0399-077X(85)80323-1. Epub 2005 Jun 20.
The authors have tested 118 stools by five techniques used for the detection of rotaviruses in human faeces : electron microscopy, latex agglutination (Slidex Rota-kit : BioMérieux ; Rotalex : Orion Diagnostica) and ELISA (Enzygnost : Behring ; Bio-Enza-Bead : Bionetics). Reproducibility and specificity for all techniques were satisfactory. Comparison of the methods showed Enzygnost to be the most sensitive (89 %), followed by the two agglutination tests, then electron microscopy and least sensitive the Bio-Enza-Bead (56 %). For large - scale screening of faeces, it appeared that the latex agglutination test were preferable for reasons of simplicity, speed and cost.
作者采用五种用于检测人类粪便中轮状病毒的技术,对118份粪便样本进行了检测,这五种技术分别是:电子显微镜检查、乳胶凝集试验(Slidex Rota-kit:生物梅里埃公司;Rotalex:奥立安诊断公司)和酶联免疫吸附测定(Enzygnost:贝林公司;Bio-Enza-Bead:Bionetics公司)。所有技术的重现性和特异性均令人满意。方法比较显示,Enzygnost最为灵敏(89%),其次是两种凝集试验,然后是电子显微镜检查,最不灵敏的是Bio-Enza-Bead(56%)。对于粪便的大规模筛查,出于操作简便、速度快和成本低的原因,乳胶凝集试验似乎更为可取。