Lukowicz G, Mounier M, Leboutet M J, Denis F, Goudeau A
Pathol Biol (Paris). 1985 Jun;33(6):681-3.
We have sought the presence of rotavirus in 237 fecal samples. Three techniques were applied to each sample: ELISA, latex agglutination (LTX) with two reagents (Rotalex and SlidexRotaKit), and electron microscopy after negative staining. The specificity of ELISA and LTX is very good (more than 93.4 per cent), the sensitivity is decreasing from ELISA to SlidexRotaKit and Rotalex (respectively 83.6-74.5 and 67.3 per cent). The advantages of LTX include rapidity, simplicity, low cost price.
我们对237份粪便样本进行了轮状病毒检测。对每份样本都应用了三种技术：酶联免疫吸附测定（ELISA）、使用两种试剂（Rotalex和SlidexRotaKit）的乳胶凝集试验（LTX）以及负染后的电子显微镜检查。ELISA和LTX的特异性非常好（超过93.4%），灵敏度从ELISA到SlidexRotaKit和Rotalex依次降低（分别为83.6% - 74.5%和67.3%）。LTX的优点包括快速、简便、成本低。
