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携带ColE1质粒的噬菌斑形成λ噬菌体的分离与鉴定

Isolation and characterization of a plaque-forming lambda bacteriophage carrying a ColE1 plasmid.

作者信息

Mukai T, Ohkubo H, Shimada K, Takagi Y

出版信息

J Bacteriol. 1978 Jul;135(1):171-7. doi: 10.1128/jb.135.1.171-177.1978.

DOI:10.1128/jb.135.1.171-177.1978
PMID:670149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC224800/
Abstract

A plaque-forming lambdaimm434 bacteriophage carrying the entire genome of colicinogenic factor E1 has been isolated and characterized. This phage, lambdaimm434ColE1, can lysogenize as a stable plasmid within a recombination-deficient Escherichia coli cell that lacks the normal attachment site for lambda phage. Furthermore, it has been found that lambdaimm434ColE1 phage carrying amber mutations in the O and P genes of the lambda genome, i.e., lambdaimm434OamPamColE1, behaves as a plaque-forming phage, and this finding suggests that the ColE1 factor DNA permits replication of the DNA of the plaque-forming phage.

摘要

一种携带产大肠杆菌素因子E1完整基因组的噬菌斑形成λimm434噬菌体已被分离并进行了特性分析。这种噬菌体，即λimm434ColE1，可作为一种稳定质粒在缺乏λ噬菌体正常附着位点的重组缺陷型大肠杆菌细胞中溶原化。此外，还发现携带λ基因组O和P基因琥珀突变的λimm434ColE1噬菌体，即λimm434OamPamColE1，表现为噬菌斑形成噬菌体，这一发现表明ColE1因子DNA允许噬菌斑形成噬菌体的DNA进行复制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faee/224800/845d507fbd4f/jbacter00290-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faee/224800/3f9c4d4f80cf/jbacter00290-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faee/224800/845d507fbd4f/jbacter00290-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faee/224800/3f9c4d4f80cf/jbacter00290-0181-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/faee/224800/845d507fbd4f/jbacter00290-0182-a.jpg

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Isolation and characterization of a plaque-forming lambda bacteriophage carrying a ColE1 plasmid.携带ColE1质粒的噬菌斑形成λ噬菌体的分离与鉴定
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本文引用的文献

1
Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication.在对DNA复制温度敏感的大肠杆菌菌株中质粒ColE1 DNA的复制。
Mol Gen Genet. 1975;136(4):273-89. doi: 10.1007/BF00341713.
2
Plasmid formation: a new mode of lysogeny by phase lambda.质粒形成：λ噬菌体溶原化的一种新模式。
Nature. 1969 Jul 12;223(5202):158-60. doi: 10.1038/223158a0.
3
Suppressor-sensitive mutants of coliphage phi-80.大肠杆菌噬菌体phi-80的抑制敏感突变体。
通过体内重组和筛选从噬菌体文库中纯化基因组序列。
Nucleic Acids Res. 1983 Apr 25;11(8):2427-45. doi: 10.1093/nar/11.8.2427.
4
Isolation and characterization of lambda phages carrying the basic replicon of the resistance plasmid R1.携带耐药质粒R1基本复制子的λ噬菌体的分离与鉴定
Mol Gen Genet. 1981;182(1):148-53. doi: 10.1007/BF00422782.
5
DNA fusion product of phage P2 with plasmid pBR322: a new phasmid.噬菌体P2与质粒pBR322的DNA融合产物：一种新的噬菌粒。
Mol Gen Genet. 1983;189(2):343-7. doi: 10.1007/BF00337829.
6
Biologically active recombinant formed through DNA pairing by purified recA protein in vitro.通过纯化的recA蛋白在体外进行DNA配对形成的具有生物活性的重组体。
Mol Gen Genet. 1983;189(2):226-34. doi: 10.1007/BF00337809.
7
ColE1 plasmid incompatibility: localization and analysis of mutations affecting incompatibility.ColE1质粒不相容性：影响不相容性的突变的定位与分析
J Bacteriol. 1979 Aug;139(2):608-19. doi: 10.1128/jb.139.2.608-619.1979.
8
Location of a ColE1 deoxyribonucleic acid region that affects the plaque-forming ability of lambda-ColE1 hybrid bacteriophage.影响λ-ColE1杂合噬菌体噬菌斑形成能力的ColE1脱氧核糖核酸区域的定位
J Bacteriol. 1979 Oct;140(1):294-6. doi: 10.1128/jb.140.1.294-296.1979.
9
Aberrant immunity behaviour of hybrid lambda imm21 phages containing the DNA of ColE1-type plasmids.含有ColE1型质粒DNA的杂交λimm21噬菌体的异常免疫行为。
Mol Gen Genet. 1979;172(3):329-37. doi: 10.1007/BF00271733.
Virology. 1968 Apr;34(4):637-49.
4
Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early functions.噬菌体DNA的复制。I. 早期功能有缺陷的λ噬菌体DNA的复制
J Mol Biol. 1968 Dec 14;38(2):217-25. doi: 10.1016/0022-2836(68)90407-5.
5
Lambda mutants which persist as plasmids.以质粒形式持续存在的λ突变体。
J Virol. 1970 Aug;6(2):218-25. doi: 10.1128/JVI.6.2.218-225.1970.
6
Prophage P1, and extrachromosomal replication unit.原噬菌体P1，以及染色体外复制单元。
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7
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Temperature-sensitive mutants for the replication of plasmids in Escherichia coli: requirement for deoxyribonucleic acid polymerase I in the replication of the plasmid ColE 1 .大肠杆菌中质粒复制的温度敏感突变体：质粒ColE1复制中对脱氧核糖核酸聚合酶I的需求
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