Aviram I, Wittenberg A, Wittenberg J B
J Biol Chem. 1978 Aug 25;253(16):5685-9.
Ferrous leghemoglobin reacts with hydrogen peroxide to form the stable product, leghemoglobin(IV). The reaction follows second order kinetics (k = 2.24 X 10(4) M-1 S-1 at 20 degrees C) and may be regarded as a single-step, two-electron oxidation. Ferric leghemoglobin is not an intermediate. The oxidation state of leghemoglobin(IV) is established by reductive titration with dithionite; 2 eq of dithionite are required to convert 1 mol of leghemoglobin(IV) to ferrous leghemoglobin. An outstanding property of leghemoglobin(IV) is its stability, little change is noted after 12 h at 25 degrees C. Leghemoglobin(IV) differs from the higher oxidation states of other hemoglobins and myoglobins in that it does not react with hydrogen peroxide to form the oxygenated protein.
亚铁豆血红蛋白与过氧化氢反应形成稳定产物豆血红蛋白（IV）。该反应遵循二级动力学（20℃时k = 2.24×10⁴ M⁻¹ s⁻¹），可视为一步两电子氧化反应。高铁豆血红蛋白不是中间体。豆血红蛋白（IV）的氧化态通过连二亚硫酸盐还原滴定确定；将1摩尔豆血红蛋白（IV）转化为亚铁豆血红蛋白需要2当量的连二亚硫酸盐。豆血红蛋白（IV）的一个显著特性是其稳定性，在25℃下放置12小时后几乎没有变化。豆血红蛋白（IV）与其他血红蛋白和肌红蛋白的较高氧化态不同，它不与过氧化氢反应形成加氧蛋白。
