Loesche W J, Gusberti F, Mettraux G, Higgins T, Syed S
Infect Immun. 1983 Nov;42(2):659-67. doi: 10.1128/iai.42.2.659-667.1983.
The predominance of anaerobic bacteria in subgingival plaque samples suggests that the pocket environment is anaerobic. In the present investigation, a small oxygen tension (pO2) electrode was inserted into the base of the pocket and the pO2 was recorded. In addition, the plaque in these pockets was examined culturally and microscopically. The oxygen tension at the bottom of 36 pockets (5 to 10 mm in depth) ranged from 5 to 27 mmHg (1 mmHg congruent to 133.3 Pa) with a mean value of 13.3 mmHg. Moderate pockets (5 and 6 mm) exhibited a mean pO2 of 15.7 mmHg, which was significantly higher than the 12.0 mmHg found in the deeper pockets. The deep pockets had higher percentages of spirochetes and Bacteroides intermedius, whereas the moderate pockets had elevated proportions of Actinomyces naeslundii and Streptococcus mutans. The sites with oxygen tensions equal to or less than 15 mmHg had significantly higher percentages of spirochetes, whereas the microaerophilic Capnocytophaga species were found in pockets with a pO2 greater than 15 mmHg. The presence of bleeding in the pocket was associated with higher proportions of B. intermedius, Capnocytophaga sp., and A. naeslundii. These pO2 readings of periodontal pockets indicated that there is a spectrum of pO2 values which seem to define, in a general way, the microbiological composition of the pocket.
龈下菌斑样本中厌氧菌占主导，这表明牙周袋环境是厌氧的。在本研究中，将一个小型氧分压（pO₂）电极插入牙周袋底部并记录pO₂。此外，对这些牙周袋中的菌斑进行了培养和显微镜检查。36个牙周袋（深度为5至10毫米）底部的氧分压范围为5至27毫米汞柱（1毫米汞柱相当于133.3帕斯卡），平均值为13.3毫米汞柱。中度牙周袋（5和6毫米）的平均pO₂为15.7毫米汞柱，显著高于较深牙周袋中的12.0毫米汞柱。较深牙周袋中螺旋体和中间普氏菌的比例更高，而中度牙周袋中内氏放线菌和变形链球菌的比例升高。氧分压等于或低于15毫米汞柱的部位螺旋体比例显著更高，而嗜二氧化碳噬纤维菌属的微需氧菌则存在于pO₂大于15毫米汞柱的牙周袋中。牙周袋出血与中间普氏菌、嗜二氧化碳噬纤维菌属和内氏放线菌的较高比例相关。这些牙周袋的pO₂读数表明，存在一系列pO₂值，这些值似乎总体上定义了牙周袋的微生物组成。
