Mirovsky Y, Halperin N, Copeliovitch L
Arch Orthop Trauma Surg (1978). 1984;102(4):238-41. doi: 10.1007/BF00436136.
To investigate the extent of local penetration of antibiotic into the bone, a single dose of 10 mg/kg gentamicin sulphate was injected over the midshaft of the left femur in 12 young and 14 old rats. In some of the rats the injected gentamicin was dissolved in saline while in the others it was dissolved in dimethylsulphoxide (DMSO). Up to 120 minutes after the injection gentamicin concentrations were measured by the fluoroimmunoassay method in the serum, cortex and spongiosa of both femurs. In young rats gentamicin was shown to have penetrated directly into the bone and its presence was limited to the femoral cortex. In the old rats, on the other hand hematogenic was found to be the antibiotic spread in the femurs. No significant difference was observed between DMSO and saline as solvents in facilitating gentamicin penetration into the bone or capillaries in either age group.
为研究抗生素在骨内的局部渗透程度，给12只年轻大鼠和14只老年大鼠的左股骨骨干中部注射单剂量10mg/kg硫酸庆大霉素。部分大鼠注射的庆大霉素溶于生理盐水，其余大鼠注射的庆大霉素溶于二甲基亚砜（DMSO）。注射后120分钟内，采用荧光免疫分析法测定双侧股骨血清、皮质和松质中的庆大霉素浓度。结果显示，年轻大鼠中庆大霉素直接渗透入骨，且仅存在于股骨皮质。另一方面，在老年大鼠中，发现庆大霉素通过血行在股骨中扩散。在促进庆大霉素渗透入骨或毛细血管方面，DMSO和生理盐水作为溶剂在两个年龄组中均未观察到显著差异。
