Mbuyi-Muamba J M, Dequeker J
Arch Orthop Trauma Surg (1978). 1984;102(4):267-72. doi: 10.1007/BF00436142.
Using EDTA extraction and collagenase digestion, cancellous bone from the femoral heads of ten normal and eight osteoarthrotic individuals was analyzed for its content of collagen, sialoprotein, proteoglycan, and carbohydrate. The EDTA extractability of the matrix proteins of the osteoarthrotic bone was significantly increased (P less than 0.001), as was the soluble collagenase-resistant fraction (SCRF). EDTA residues, bone matrix size, the collagenase-resistant fraction (CRF), and the insoluble collagenase-resistant fraction (ICRF) of the osteoarthrotic cases were not different from those of the controls. The amounts of carbohydrate and proteoglycans were considerably elevated in the bone matrix of the osteoarthrotic bone (P less than 0.01 and P less than 0.001, respectively). In the EDTA extracts, sialoprotein and proteoglycan contents were found in significant higher amounts (P less than 0.05 and P less than 0.01, respectively) in the osteoarthrotic cases. In the SCRF, the hexose and sialic acid contents were higher in the osteoarthrotic bone (P less than 0.01), while in the ICRF all the analyses were significantly increased in the osteoarthrotic bone (P less than 0.001). The ratios of collagen to non-collagenous components were lower in the osteoarthrotic than in normal bones. The quantitative and qualitative variations in cancellous bone proteins from the femoral head in osteoarthrosis found in this study suggest that alterations in subchondral bone play a role in the pathophysiology of cartilage degeneration in osteoarthrosis.
采用乙二胺四乙酸（EDTA）提取法和胶原酶消化法，对10名正常人和8名骨关节炎患者股骨头的松质骨进行分析，测定其胶原、涎蛋白、蛋白聚糖和碳水化合物的含量。骨关节炎患者骨基质蛋白的EDTA可提取性显著增加（P<0.001），可溶性抗胶原酶组分（SCRF）也是如此。骨关节炎患者的EDTA残留量、骨基质大小、抗胶原酶组分（CRF）和不溶性抗胶原酶组分（ICRF）与对照组无差异。骨关节炎患者骨基质中的碳水化合物和蛋白聚糖含量显著升高（分别为P<0.01和P<0.001）。在EDTA提取物中，骨关节炎患者的涎蛋白和蛋白聚糖含量显著更高（分别为P<0.05和P<0.01）。在SCRF中，骨关节炎患者骨中的己糖和唾液酸含量更高（P<0.01），而在ICRF中，骨关节炎患者骨中的所有分析指标均显著增加（P<0.001）。骨关节炎患者骨中胶原与非胶原成分的比例低于正常骨。本研究中发现的骨关节炎患者股骨头松质骨蛋白的定量和定性变化表明，软骨下骨的改变在骨关节炎软骨退变的病理生理学中起作用。
