Schwartz E R, Leveille C R, Stevens J W, Oh W H
Arthritis Rheum. 1981 Dec;24(12):1528-39. doi: 10.1002/art.1780241212.
Sulfated proteoglycan structure and metabolism in osteoarthritic articular cartilage from the hind-limb joints of guinea pigs in which the disease had been surgically induced were evaluated and compared with those from nonoperated or sham-operated controls. In these studies, conducted on animals receiving high and low dietary levels of ascorbic acid, the effect of vitamin C on these variables was also examined. Sulfated proteoglycan biosynthesis per unit of DNA as reflected by in vivo uptake of 35S-sulfate was stimulated both by arthritic development and high dietary vitamin C. Under these conditions, the specific activities, 35S-cpm/micrograms uronic acid, of the proteoglycans were elevated. Neither arthritic development nor variation in dietary levels of vitamin C altered the structure of the proteoglycans. Two species of proteoglycans that exhibited the same electrophoretic mobilities in agarose/acrylamide gels, the same elution profiles on high performance liquid chromatography,, and the same galactosamine/glucosamine ratios were found in all normal and osteoarthritic cartilage specimens tested. The specific activities of these two specimens tested. The specific activities of these two species were dissimilar. In all samples, the larger of the two species had a higher specific activity.
对通过手术诱导患骨关节炎的豚鼠后肢关节的骨关节炎性关节软骨中硫酸化蛋白聚糖的结构和代谢进行了评估，并与未手术或假手术对照组的软骨进行了比较。在这些对摄入高、低膳食水平抗坏血酸的动物进行的研究中，还检测了维生素C对这些变量的影响。骨关节炎的发展和高膳食维生素C均刺激了以35S-硫酸盐的体内摄取所反映的每单位DNA的硫酸化蛋白聚糖生物合成。在这些条件下，蛋白聚糖的比活性（35S-每分钟计数/微克糖醛酸）升高。骨关节炎的发展和维生素C膳食水平的变化均未改变蛋白聚糖的结构。在所有测试的正常和骨关节炎软骨标本中，发现了两种在琼脂糖/丙烯酰胺凝胶中具有相同电泳迁移率、在高效液相色谱中具有相同洗脱谱以及相同半乳糖胺/葡萄糖胺比率的蛋白聚糖。测试的这两个标本的比活性不同。在所有样品中，两种蛋白聚糖中较大的一种具有较高的比活性。
