York D, Holt S, Rothwell N, Stock M
Am J Physiol. 1984 May;246(5 Pt 1):E391-6. doi: 10.1152/ajpendo.1984.246.5.E391.
Experiments were performed to investigate the effect of age and genotype on brown adipose tissue thermogenesis in Zucker rats. Specific [3H]GDP binding to interscapular brown adipose tissue mitochondria ( IBATM ) was reduced in 14-day-old preobese fa/fa rats and remained lower after weaning. A gene-dosage effect of the recessive fa gene was observed in 8- to 10-wk-old rats in both IBATM [3H]GDP binding and the thermic effect of a balanced meal (50 kJ Complan ), measured by indirect calorimetry. In each case the heterozygote (Fa/fa) group had a value intermediate between those of obese (fa/fa) and the homozygous lean (Fa/Fa) groups. Norepinephrine increased IBATM [3H]GDP binding to similar levels in lean (Fa/fa) and obese (fa/fa) rats and induced similar increases in oxygen consumption in Fa/Fa, Fa/fa, and fa/fa rats. It is concluded that the impaired, diet-related brown adipose tissue thermogenesis is closely related to the primary gene defect in the obese rat. This defect may result from misregulation of the autonomic nervous system.
进行实验以研究年龄和基因型对Zucker大鼠棕色脂肪组织产热的影响。在14日龄的肥胖前期fa/fa大鼠中，肩胛间棕色脂肪组织线粒体（IBATM）上特异性[3H]GDP结合减少，断奶后仍保持较低水平。在8至10周龄的大鼠中，无论是在IBATM [3H]GDP结合还是通过间接量热法测量的均衡膳食（50 kJ Complan）的热效应方面，均观察到隐性fa基因的基因剂量效应。在每种情况下，杂合子（Fa/fa）组的值介于肥胖（fa/fa）组和纯合瘦型（Fa/Fa）组之间。去甲肾上腺素使瘦型（Fa/fa）和肥胖（fa/fa）大鼠的IBATM [3H]GDP结合增加到相似水平，并在Fa/Fa、Fa/fa和fa/fa大鼠中诱导相似的耗氧量增加。得出的结论是，与饮食相关的棕色脂肪组织产热受损与肥胖大鼠的原发性基因缺陷密切相关。这种缺陷可能是由自主神经系统的调节异常导致的。
