用于检测组织培养中支原体污染的克隆支原体核糖体RNA基因。
Cloned mycoplasma ribosomal RNA genes for the detection of mycoplasma contamination in tissue cultures.
作者信息
Göbel U B, Stanbridge E J
出版信息
Science. 1984 Dec 7;226(4679):1211-3. doi: 10.1126/science.6505688.
PMID:6505688
Abstract
A cloned fragment of the mycoplasma ribosomal RNA operon was used as a molecular probe for the detection of mycoplasmas in cell cultures. According to the conditions of hybridization, the probe can detect prokaryotes in general or mycoplasmas specifically.
摘要
支原体核糖体RNA操纵子的一个克隆片段被用作分子探针，用于检测细胞培养物中的支原体。根据杂交条件，该探针可以一般地检测原核生物，也可以特异性地检测支原体。
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