McGarrity G J, Kotani H
Exp Cell Res. 1986 Mar;163(1):273-8. doi: 10.1016/0014-4827(86)90581-1.
The utility of a genetic probe prepared against mycoplasmal ribosomal RNA was evaluated. The probe consisted of a tritiated DNA probe prepared with reverse transcriptase against Mycoplasma and Acholeplasma. Using a batch process without Southern blots and autoradiography, the probe assay detected mycoplasma infection in 27 of 52 cell cultures, an incidence of 51.9%. No false-positives were detected. Two false-negatives occurred; these were unusual mycoplasma infections of cell cultures grown in serum-free media. The sensitivity of the probe varied with mycoplasma species, from 9.3 X 10(3) for M. arginini to 2.6 X 10(6) for M. orale. The assay is rapid, taking only 2-3 h.
对一种针对支原体核糖体RNA制备的基因探针的效用进行了评估。该探针由用逆转录酶针对支原体和无胆甾原体制备的氚标记DNA探针组成。使用无需Southern印迹和放射自显影的批量方法，探针检测法在52个细胞培养物中的27个中检测到支原体感染，发生率为51.9%。未检测到假阳性。出现了两例假阴性；这些是在无血清培养基中生长的细胞培养物的不寻常支原体感染。探针的敏感性因支原体种类而异，从精氨酸支原体的9.3×10³到口腔支原体的2.6×10⁶。该检测方法快速，仅需2 - 3小时。
