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一种用于检测鸡败血支原体的种特异性DNA探针。

A species-specific DNA probe for the detection of Mycoplasma gallisepticum.

作者信息

Santha M, Burg K, Rasko I, Stipkovits L

机构信息

Veterinary Medical Research Institute, Hungarian Academy of Sciences, Budapest.

出版信息

Infect Immun. 1987 Nov;55(11):2857-9. doi: 10.1128/iai.55.11.2857-2859.1987.

DOI:10.1128/iai.55.11.2857-2859.1987
PMID:3666965
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259991/
Abstract

An 800-base-pair DNA fragment from a partial genomic library of Mycoplasma gallisepticum was selected and used as a probe for the selective detection of this avian pathogen. The specificity and sensitivity of this probe were demonstrated by using dot blot and Southern hybridizations.

摘要

从鸡败血支原体部分基因组文库中选取了一个800个碱基对的DNA片段，并将其用作探针，用于选择性检测这种禽病原体。通过斑点印迹和Southern杂交证明了该探针的特异性和敏感性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/72853f8f44ff/iai00095-0328-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/ff15a617a064/iai00095-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/37fe5bc93994/iai00095-0328-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/72853f8f44ff/iai00095-0328-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/ff15a617a064/iai00095-0327-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/37fe5bc93994/iai00095-0328-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f5a/259991/72853f8f44ff/iai00095-0328-b.jpg

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本文引用的文献

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Quantitative binding of covalently closed circular DNA to nitrocellulose in NaI.共价闭合环状DNA在碘化钠中与硝酸纤维素的定量结合。
Anal Biochem. 1983 Mar;129(2):357-64. doi: 10.1016/0003-2697(83)90562-6.
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Detection of mycoplasmas infecting cell cultures by DNA hybridization.通过DNA杂交检测感染细胞培养物的支原体。
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Science. 1984 Dec 7;226(4679):1211-3. doi: 10.1126/science.6505688.
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A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.一种将DNA限制性内切酶片段放射性标记至高比活度的技术。
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Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.用于可视化与固定在硝酸纤维素膜上的DNA或RNA杂交的生物素标记DNA探针的快速灵敏比色法：生物印迹法。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045-9. doi: 10.1073/pnas.80.13.4045.
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Selective detection of Mycoplasma hyorhinis using cloned genomic DNA fragments.使用克隆的基因组DNA片段选择性检测猪鼻支原体。
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Molecular biology and genetics of mycoplasmas (Mollicutes).支原体（柔膜菌纲）的分子生物学与遗传学
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