通过生物发光检测菌尿：样本分析前离心的影响
Detection of bacteriuria by bioluminescence: effect of pre-analysis centrifugation of specimens.
作者信息
Nichols W W, Curtis G D, Johnston H H
出版信息
J Appl Bacteriol. 1984 Apr;56(2):247-57. doi: 10.1111/j.1365-2672.1984.tb01345.x.
Three bioluminescence-based, rapid methods of detecting significant bacteriuria were applied in parallel to 514 urine specimens. The results were compared with those of a quantitative pour plate viable count method, defined as positive if greater than or equal to 10(5) c.f.u./ml of urine were observed. When adjusted to yield 21% falsely positive results the three rapid methods yielded 24%, 21% and 19% falsely negative results. If specimens with evidence of urethral or vaginal contamination were excluded (237 specimens remaining) the three methods yielded respectively 14%, 8% and 13% falsely negative results. A major source of disagreement between the bioluminescence-based methods and quantitative culture thus appeared to be contaminated urine specimens.
三种基于生物发光的快速检测显著菌尿的方法同时应用于514份尿液标本。将结果与定量倾注平板活菌计数法的结果进行比较，若观察到每毫升尿液中菌落形成单位（c.f.u.）大于或等于10(5)，则定量倾注平板活菌计数法判定为阳性。当调整至产生21%的假阳性结果时，这三种快速方法分别产生了24%、21%和19%的假阴性结果。如果排除有尿道或阴道污染迹象的标本（剩余237份标本），这三种方法分别产生了14%、8%和13%的假阴性结果。因此，基于生物发光的方法与定量培养之间存在分歧的一个主要来源似乎是受污染的尿液标本。
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