成年大鼠中的神经发生：光镜放射自显影片的电子显微镜分析
Neurogenesis in the adult rat: electron microscopic analysis of light radioautographs.
作者信息
Kaplan M S, Hinds J W
出版信息
Science. 1977 Sep 9;197(4308):1092-4. doi: 10.1126/science.887941.
PMID:887941
Abstract
Three-month-old rats were injected intraperitoneally with [3H]thymidine (4.3 microcuries per gram of body weight) and allowed to survive for 30 days. Radioautography of 1-micrometer sections revealed labeled cells in the granular layers of dentate gyrus and olfactory bulb; these were confirmed as neurons by electron microscopy of reembedded 1-micrometer sections.
摘要
给3个月大的大鼠腹腔注射[3H]胸腺嘧啶核苷（每克体重4.3微居里），并使其存活30天。对1微米厚的切片进行放射自显影，发现在齿状回和嗅球的颗粒层中有标记细胞；通过对重新包埋的1微米厚切片进行电子显微镜检查，证实这些细胞为神经元。
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