Rutter J M, Luther P D
Vet Rec. 1984 Apr 21;114(16):393-6. doi: 10.1136/vr.114.16.393.
A toxin produced by strains of Pasteurella multocida isolated from pigs with atrophic rhinitis caused a cytopathic effect in cell cultures derived from embryonic bovine lung. The toxin was produced during the late logarithmic phase of bacterial growth and inactivated by heating for 30 minutes at 56 degrees C. The cell culture assay was reproducible and 10(3) to 10(4) times more sensitive than a lethal assay in BALB/c mice. There was complete agreement between results in the two tests with 76 isolates of P multocida. Neutralising activity was demonstrated in both assays with sera from infected gnotobiotic piglets. It was concluded that embryonic bovine lung cell cultures provided a sensitive in vitro test for the differentiation of toxigenic from non toxigenic isolates of P multocida. The assay could be used in diagnostic laboratories and for characterisation of the toxin.
从患有萎缩性鼻炎的猪中分离出的多杀性巴氏杆菌菌株产生的一种毒素，对源自胚胎牛肺的细胞培养物产生了细胞病变效应。该毒素在细菌生长的对数后期产生，并在56℃加热30分钟后失活。细胞培养测定具有可重复性，比BALB/c小鼠的致死测定灵敏度高10³至10⁴倍。对76株多杀性巴氏杆菌进行的两项试验结果完全一致。在两种测定中，感染无菌仔猪的血清均显示出中和活性。得出的结论是，胚胎牛肺细胞培养为区分多杀性巴氏杆菌产毒菌株和非产毒菌株提供了一种灵敏的体外试验。该测定可用于诊断实验室和毒素的特性鉴定。
