Dimitrova E
Vet Med Nauki. 1984;21(1):17-21.
The use of Nobuto 's method for blood sampling with birds and mammals, using filter paper, suggests various advantages in the practice of serologic investigations. Studies were carried out to determine the immune titer of birds against Newcastle disease. Parallel blood samples were taken routinely and after the method of Nobuto with filter paper. Serologic examination was performed through the hemagglutination-inhibition test, beta procedure, with 10 HAU /0.5 cm3. As many as five parallel experiments with blood samples from birds were conducted within the period of from the 15th to the 40th day following vaccination. The results of HI-tests on filter paper, variants a and b, proved mathematically reliable, coinciding with the HI results with the samples studied with sera (P = 0.05). Further studies of blood samples from birds after the HI, beta micromethod, are currently carried out.
野藤（Nobuto）使用滤纸对鸟类和哺乳动物进行采血的方法，在血清学调查实践中显示出多种优势。开展了多项研究以确定鸟类针对新城疫的免疫效价。常规采集平行血样，并按照野藤方法使用滤纸采集血样。通过血凝抑制试验（β程序，10个血凝单位/0.5立方厘米）进行血清学检查。在接种疫苗后的第15天至第40天期间，对鸟类血样进行了多达五次平行实验。滤纸变体a和b上的血凝抑制试验结果经数学验证可靠，与血清样本的血凝抑制结果相符（P = 0.05）。目前正在对采用血凝抑制β微量法检测后的鸟类血样进行进一步研究。
