Watts R P, Kim H D
Biol Neonate. 1984;45(6):280-8. doi: 10.1159/000242018.
A four-fold increase in red blood cell 2,3-diphosphoglycerate (DPG) levels occurs within 1-2 weeks after birth resulting in a decrease in oxygen affinity in the newborn pig. In this study, we found that although newborn pig red blood cells seem to be equally capable of net DPG synthesis as adult pig cells when challenged by a mixture of inosine, pyruvate and phosphate, the cells from newborns degrade DPG much faster than do cells from adults. As a result, DPG levels in the newborn's cells do not seem to increase in comparison to the adult's cells. Using a density centrifugation technique, it was found that the postnatal cells initially entering the circulation have a relatively low rather than high DPG content. However, DPG content quickly increases as they age. These findings suggest that the rapid rise of DPG seen in the growing piglet is brought about in part (a) by an elimination of the fetal cells with low DPG, and (b) by an emergence of postnatal cells having the capacity to augment their DPG content while in circulation.
出生后1 - 2周内，新生仔猪红细胞中的2,3 - 二磷酸甘油酸（DPG）水平会增加四倍，导致新生仔猪的氧亲和力降低。在本研究中，我们发现，尽管当受到肌苷、丙酮酸和磷酸盐混合物刺激时，新生仔猪红细胞合成DPG的净能力似乎与成年猪细胞相当，但新生仔猪的细胞降解DPG的速度比成年猪的细胞快得多。因此，与成年猪细胞相比，新生仔猪细胞中的DPG水平似乎没有增加。使用密度离心技术发现，最初进入循环的产后细胞DPG含量相对较低而非较高。然而，随着它们的老化，DPG含量会迅速增加。这些发现表明，生长中的仔猪中DPG的快速升高部分是由于（a）低DPG的胎儿细胞被清除，以及（b）具有在循环中增加其DPG含量能力的产后细胞的出现。
