Adler T R, Beall G N, Curd J G, Heiner D C, Sabharwal U K
Clin Exp Immunol. 1984 May;56(2):383-9.
Using a sensitive radioimmunoassay, it was demonstrated that autoantibodies to human thyroglobulin (hTg) did not activate complement upon interaction with solid phase adsorbed hTg. Since IgG4 immunoglobulins do not activate complement, we studied sera containing large amounts of anti-thyroglobulin (anti-Tg) with a sensitive radioimmunoassay for IgG4 anti-Tg. Antibodies of the IgG4 subclass were detected in the sera of each of the eight patients studied and not in control subjects. Quantitative assays of IgG4 anti-Tg were performed by comparing the removal of anti-Tg and total IgG4 during immunoadsorption of anti-Tg. The contribution of IgG4 anti-Tg antibodies to the total quantity of anti-Tg ranged from 6 to 30%, an amount inadequate to explain the lack of complement activation by anti-Tg.
采用灵敏的放射免疫分析法表明，人甲状腺球蛋白（hTg）自身抗体与固相吸附的hTg相互作用时不会激活补体。由于IgG4免疫球蛋白不会激活补体，我们用灵敏的IgG4抗Tg放射免疫分析法研究了含有大量抗甲状腺球蛋白（抗Tg）的血清。在所研究的8例患者的血清中均检测到了IgG4亚类抗体，而对照受试者血清中未检测到。通过比较抗Tg免疫吸附过程中抗Tg和总IgG4的去除情况，对IgG4抗Tg进行定量分析。IgG4抗Tg抗体在抗Tg总量中的占比为6%至30%，这一数量不足以解释抗Tg缺乏补体激活的现象。
