Mann R C, Popp D M, Hand R E
Cytometry. 1984 May;5(3):304-7. doi: 10.1002/cyto.990050314.
In order to visualize multiparameter flow cytometric measurements it is desirable to reduce the dimensionality of the data to two while preserving important features of each data pattern. We describe a method that projects two-parameter data onto a straight line that forms an angle theta with one of the original parameter axis. The angle is selected interactively or by principal component analysis. The procedure is applied on-line or off-line to pairs of parameters of the original data set that has been collected in LIST mode.
