Izaki Y, Yoshikawa S, Uchiyama M
Lipids. 1984 May;19(5):324-31. doi: 10.1007/BF02534782.
Thermally oxidized rapeseed oils (4 levels of deterioration; used by a manufacturer of fried fish paste in a conventional manner) were fed to rats at a practical level of concentration. Rats were fed a diet ad libitum for 13 weeks that contained 15% of a test oil. The effects of the diet on several biochemical criteria related to peroxidative alterations were investigated. In groups given thermally oxidized oils relative liver weight, relative kidney weight, thiobarbituric acid-reactive substances (TBA-RS) in the liver and reduced glutathione content were increased significantly in proportion to the degree of deterioration of the oil, compared with the group given fresh oil. Tocopherol contents in both serum and liver were decreased considerably in proportion to the deterioration level of the supplied oils. The above criteria correlated well with various deterioration indices of the oil. For instance, TBA-RS was well correlated (p less than 0.001) with petroleum ether-insoluble oxidized fatty acid (r = 0.9191), column chromatographically separated polar fraction (r = 0.9056), glyceride dimer fraction (r = 0.9023) and carbonyl value (r = 0.8647).
将热氧化菜籽油（4个变质水平；由一家炸鱼酱制造商以传统方式使用）以实际浓度水平喂给大鼠。大鼠自由采食含15%试验油的日粮13周。研究了该日粮对与过氧化改变相关的几个生化指标的影响。与喂食新鲜油的组相比，在喂食热氧化油的组中，相对肝脏重量、相对肾脏重量、肝脏中的硫代巴比妥酸反应性物质（TBA-RS）以及还原型谷胱甘肽含量均随着油的变质程度显著增加。血清和肝脏中的生育酚含量随着所供应油的变质水平而显著降低。上述指标与油的各种变质指数密切相关。例如，TBA-RS与石油醚不溶性氧化脂肪酸（r = 0.9191）、柱色谱分离的极性部分（r = 0.9056）、甘油二酯部分（r = 0.9023）和羰基值（r = 0.8647）密切相关（p < 0.001）。
