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来自鸡的卵清蛋白信使核糖核酸5'端的核苷酸序列。

Nucleotide sequence at the 5' end of ovalbumin messenger RNA from chicken.

作者信息

Kuebbing D, Liarakos C D

出版信息

Nucleic Acids Res. 1978 Jul;5(7):2253-66. doi: 10.1093/nar/5.7.2253.

DOI:10.1093/nar/5.7.2253
PMID:673854
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342161/
Abstract

DNA-sequence analysis of 300 nucleotides from the region of cloned, double-stranded ovalbumin cDNA corresponding to the 5' end of ovalbumin messenger RNA was accomplished using the technique of Maxam and Gilbert (Proc. Nat. Acad. Sci. USA (1977) 74,560-564). The AUG initiation codon was located 52 nucleotides from the AT linkers used in cloning and immediately adjacent to the amino terminal peptide of ovalbumin, indicating the absence of a "signal peptide" in this protein. The nucleotide sequence coding for a phosphorylated peptide from ovalbumin was also found. These results demonstrate that the coding portion of mRNAov begins near the 5' end of the molecule leaving some 600 nucleotides of noncoding information at the 3' end.

摘要

利用马克萨姆和吉尔伯特的技术（《美国国家科学院院刊》（1977年）74卷，560 - 564页），对克隆的双链卵清蛋白cDNA中对应于卵清蛋白信使RNA 5'端区域的300个核苷酸进行了DNA序列分析。AUG起始密码子位于克隆时所用AT连接子下游52个核苷酸处，且紧邻卵清蛋白的氨基末端肽段，这表明该蛋白不存在“信号肽”。还发现了编码卵清蛋白磷酸化肽段的核苷酸序列。这些结果表明，卵清蛋白信使RNA的编码部分始于分子的5'端附近，在3'端留下约600个核苷酸的非编码信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/a7e0cb78cd3f/nar00468-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/0fbeb8793154/nar00468-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/974d045172fc/nar00468-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/f3736d491dd5/nar00468-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/3f6583112877/nar00468-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/a7e0cb78cd3f/nar00468-0049-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/0fbeb8793154/nar00468-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/974d045172fc/nar00468-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/f3736d491dd5/nar00468-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/3f6583112877/nar00468-0048-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b58e/342161/a7e0cb78cd3f/nar00468-0049-a.jpg

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Nucleotide sequence at the 5' end of ovalbumin messenger RNA from chicken.来自鸡的卵清蛋白信使核糖核酸5'端的核苷酸序列。
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引用本文的文献

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本文引用的文献

1
A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.一种制备高比活度32-P标记三磷酸腺苷的简单方法。
Biochem J. 1964 Jan;90(1):147-9. doi: 10.1042/bj0900147.
2
An application of diagonal electrophoresis to the selective purification of serine phosphate peptides. Serine phosphate peptides from ovalbumin.对角线电泳在磷酸丝氨酸肽选择性纯化中的应用。来自卵清蛋白的磷酸丝氨酸肽。
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Improved estimation of secondary structure in ribonucleic acids.
单纯疱疹病毒扩增子：大小对含有真核DNA序列的构建缺陷基因组复制的影响。
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Characterization of the effects on ovalbumin mRNA of aminomethyl-trimethylpsoralen photoreaction with hen oviduct mRNA.氨甲基三甲基补骨脂素与鸡输卵管mRNA光反应对卵清蛋白mRNA影响的表征。
Nucleic Acids Res. 1987 Oct 26;15(20):8417-38. doi: 10.1093/nar/15.20.8417.
5
Determination of secondary structure in the initiation region of ovalbumin mRNA.卵清蛋白mRNA起始区域二级结构的测定
Nucleic Acids Res. 1988 Dec 9;16(23):11249-65. doi: 10.1093/nar/16.23.11249.
6
Intermolecular base-paired interaction between complementary sequences present near the 3' ends of 5S rRNA and 18S (16S) rRNA might be involved in the reversible association of ribosomal subunits.5S rRNA和18S（16S）rRNA 3'端附近存在的互补序列之间的分子间碱基配对相互作用可能参与核糖体亚基的可逆结合。
Nucleic Acids Res. 1979 Dec 11;7(7):1913-29. doi: 10.1093/nar/7.7.1913.
核糖核酸二级结构的改进估计。
Nat New Biol. 1973 Nov 14;246(150):40-1. doi: 10.1038/newbio246040a0.
4
Physical and chemical characterization of purified ovalbumin messenger RNA.纯化的卵清蛋白信使核糖核酸的物理和化学特性
J Biol Chem. 1975 Sep 10;250(17):7027-39.
5
Rat liver pre-proalbumin: complete amino acid sequence of the pre-piece. Analysis of the direct translation product of albumin messenger RNA.大鼠肝脏前原白蛋白：前肽段的完整氨基酸序列。白蛋白信使核糖核酸直接翻译产物的分析。
J Biol Chem. 1977 Oct 10;252(19):6846-55.
6
Precursor of egg white lysozyme. Amino acid sequence of an NH2-terminal extension.蛋清溶菌酶的前体。NH2末端延伸的氨基酸序列。
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