电子显微镜检查和限制性内切酶图谱分析揭示了鸡卵清蛋白分裂基因中更多的间隔序列。

Electron microscopy and restriction enzyme mapping reveal additional intervening sequences in the chicken ovalbumin split gene.

作者信息

Garapin A C, Cami B, Roskam W, Kourilsky P, Le Pennec J P, Perrin F, Gerlinger P, Cochet M, Chambon P

出版信息

Cell. 1978 Jul;14(3):629-39. doi: 10.1016/0092-8674(78)90247-7.

Abstract

The Eco RI fragment "b" of chicken DNA (Breathnach, Mandel and Chambon, 1977), which contains the sequences coding for the 5' quarter of ovalbumin mRNA (ov mRNA), has been isolated by molecular cloning using a "shotgun" approach. Electron microscopy and restriction enzyme analysis have revealed that the sequences coding for the 5' quarter (approximately 500 nucleotides) of ov mRNA are split into four regions separated by three intervening sequences. The cloning procedure seems to be reliable, since the restriction enzyme pattern of the cloned Econ RI fragment "b" is similar to that of the corresponding chromosomal DNA fragment. There is no evidence supporting the existence of a 150-200 nucleotide long sequence at the 5' end of the ov mRNA similar to the "leader" sequences found at the 5' end of some adenovirus and SV40 mRNAs.

摘要

鸡DNA的Eco RI片段“b”（Breathnach、Mandel和Chambon，1977年），它包含编码卵清蛋白mRNA（ov mRNA）5'端四分之一的序列，已通过“鸟枪法”分子克隆分离出来。电子显微镜和限制性内切酶分析表明，编码ov mRNA 5'端四分之一（约500个核苷酸）的序列被分成四个区域，由三个间隔序列隔开。克隆程序似乎是可靠的，因为克隆的Eco RI片段“b”的限制性内切酶图谱与相应的染色体DNA片段相似。没有证据支持在ov mRNA的5'端存在一个150 - 200个核苷酸长的序列，类似于在一些腺病毒和SV40 mRNA的5'端发现的“前导”序列。