Doel M T, Houghton M, Cook E A, Carey N H
Nucleic Acids Res. 1977 Nov;4(11):3701-13. doi: 10.1093/nar/4.11.3701.
Chicken DNA has been digested with restriction enzymes and the size distribution of the DNA fragments containing ovalbumin specific sequences has been examined after separation of the fragments on agarose gels and transfer to nitrocellulose sheets. Hybridisation with terminally 32P-labelled ovalbumin mRNA fragments or with RNA populations transcribed from the DNA of a hybrid plasmid containing ovalbumin sequences was used to locate the DNA fragments coding for ovalbumin. Digestion with enzymes which do not cut within the portion of the ovalbumin gene synthesised from ovalbumin messenger RNA in vitro has shown the presence of several defined fragments carrying ovalbumin specific sequences. Possible explanations of these observations are discussed.
用限制性内切酶消化鸡的DNA，在琼脂糖凝胶上分离DNA片段并转移到硝酸纤维素膜上后，检测含有卵清蛋白特异性序列的DNA片段的大小分布。用末端32P标记的卵清蛋白mRNA片段或与从含有卵清蛋白序列的杂交质粒的DNA转录的RNA群体进行杂交，以定位编码卵清蛋白的DNA片段。用在体外由卵清蛋白信使RNA合成的卵清蛋白基因部分内不切割的酶进行消化，已显示存在几个携带卵清蛋白特异性序列的特定片段。讨论了这些观察结果的可能解释。
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