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一株具有异常NS基因的高细胞溶解性乙型流感病毒变体的分离与初步鉴定

Isolation and preliminary characterization of a highly cytolytic influenza B virus variant with an aberrant NS gene.

作者信息

Tanaka T, Urabe M, Goto H, Tobita K

出版信息

Virology. 1984 Jun;135(2):515-23. doi: 10.1016/0042-6822(84)90205-8.

DOI:10.1016/0042-6822(84)90205-8
PMID:6740944
Abstract

By repeated backcrosses of influenza virus A/Aichi/2/68 (H3N2) with B/Yamagata/1/73 in MDCK cells, a virus clone with HA of B serotype (clone B/610B5B/201, or clone 201) was obtained, which formed sharp plaques in MDCK cells and induced a severe cell lysis early after infection. Its structural proteins were indistinguishable from those of B/Yamagata. Electrophoresis of the RNA of the clone also showed an identical pattern to that of B/Yamagata except RNA segment 8 (NS gene), which migrated faster than the corresponding segment of B/Yamagata in a 2.8% polyacrylamide gel. Within the clone 201-infected MDCK cells, only one species of nonstructural (NS) polypeptide was demonstrable, which had the same electrophoretic mobility as NS2 of B/Yamagata, and any band which might be taken as the counterpart of NS1 of B/Yamagata was not detectable on the gel. Peptide mapping revealed that NS of clone 201 was structurally different from either NS1 or NS2 of wild-type B/Yamagata. NS gene and its function of clone 201 was successfully transferred to B/Lee/40 by genetic reassortment.

摘要

通过在MDCK细胞中使甲型流感病毒A/爱知/2/68（H3N2）与乙型流感病毒B/山形/1/73反复回交，获得了一株具有乙型血清型血凝素（HA）的病毒克隆（克隆B/610B5B/201，即克隆201），该克隆在MDCK细胞中形成清晰的蚀斑，并在感染后早期诱导严重的细胞裂解。其结构蛋白与B/山形的结构蛋白无法区分。该克隆的RNA电泳也显示出与B/山形相同的图谱，只是RNA片段8（NS基因）在2.8%聚丙烯酰胺凝胶中的迁移速度比B/山形的相应片段快。在克隆201感染的MDCK细胞中，仅可检测到一种非结构（NS）多肽，其电泳迁移率与B/山形的NS2相同，在凝胶上未检测到任何可能被视为B/山形NS1对应物的条带。肽图谱分析表明，克隆201的NS在结构上与野生型B/山形的NS1或NS2均不同。通过基因重配，克隆201的NS基因及其功能成功转移至B/李/40。

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