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丙型流感病毒重组体的分离

Isolation of influenza C virus recombinants.

作者信息

Racaniello V R, Palese P

出版信息

J Virol. 1979 Dec;32(3):1006-14. doi: 10.1128/JVI.32.3.1006-1014.1979.

DOI:10.1128/JVI.32.3.1006-1014.1979
PMID:513198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC525950/
Abstract

Recombinants between two different influenza C viruses were isolated. In MDCK (canine kidney) cells, one strain, C/JJ/50, caused lytic plaques, whereas C/JHG/66 virus did not produce clear plaques. From a mixed infection of MDCK cells with C/JHG/66 virus and UV-inactivated C/JJ/50 virus, clones were isolated which possessed the clear-plaque phenotype. Fingerprint analyses indicated that the RNAs of parent viruses had different oligonucleotide patterns and that one of the clones derived from the mixed infection was formed by reassortment of parental genes. This recombinant clone most likely inherited RNAs 1, 2, 3, 6, and 7 from C/JGH/66 virus and RNAs 4 and 5 from C/JJ/50 virus.

摘要

分离出了两种不同丙型流感病毒之间的重组体。在犬肾（MDCK）细胞中，一种毒株C/JJ/50会产生溶菌性噬斑，而C/JHG/66病毒不会产生明显的噬斑。通过用C/JHG/66病毒和紫外线灭活的C/JJ/50病毒对MDCK细胞进行混合感染，分离出了具有明显噬斑表型的克隆。指纹分析表明，亲本病毒的RNA具有不同的寡核苷酸模式，并且混合感染产生的一个克隆是由亲本基因重配形成的。这个重组克隆很可能从C/JGH/66病毒继承了RNA 1、2、3、6和7，从C/JJ/50病毒继承了RNA 4和5。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/adb8d49a633d/jvirol00192-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/b71a5e19f752/jvirol00192-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/d7051315521a/jvirol00192-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/01502d363d00/jvirol00192-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/adb8d49a633d/jvirol00192-0318-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/b71a5e19f752/jvirol00192-0314-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/d7051315521a/jvirol00192-0315-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/01502d363d00/jvirol00192-0316-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/80db/525950/adb8d49a633d/jvirol00192-0318-a.jpg

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