Pettersson P, Van R, Karlsson M, Björntorp P
Metabolism. 1985 Sep;34(9):808-12. doi: 10.1016/0026-0495(85)90103-9.
Adipose precursors isolated from the stromal-vascular fraction of omental and subcutaneous adipose tissue, from defined hyperplastic obese and nonobese human adults were cultured in order to measure and compare replication rates. After multiplication to confluence these cells were also cultured in an enriched viscous suspension medium to optimize the expression of these cells to adipocytes, allowing an estimation of the number of cells having the ability to express an adipocyte phenotype. No difference in replication rate was seen between obese and nonobese donors or when adipocyte precursors from different depots were compared. When cells were allowed to develop fully in the enriched medium, approximately 6.5% of the original inoculated cell population exhibited an adipocyte morphology. Thus, these results suggest that environmental rather than genetic factors may be responsible for the hyperplasia seen in certain massively obese humans. Furthermore, the results indicate that fat-free cells found within the stromal-vascular fraction of adipose tissue have the ability to develop into adipocytes. However, it is suggested that the relatively low yield in obtaining fully differentiated fat cells under these conditions may be due to the heterogeneity of adipose related cells within the original stromal-vascular fraction from which these cultures were initially derived.
从明确的增生性肥胖和非肥胖成年人类的网膜和皮下脂肪组织的基质血管部分分离出脂肪前体细胞，进行培养以测量和比较增殖率。这些细胞增殖至汇合后，还在富含粘性的悬浮培养基中培养，以优化这些细胞向脂肪细胞的分化，从而估计具有表达脂肪细胞表型能力的细胞数量。在肥胖和非肥胖供体之间，或者比较来自不同部位的脂肪细胞前体时，未观察到增殖率的差异。当细胞在富含营养的培养基中充分发育时，约6.5%的原始接种细胞群体呈现出脂肪细胞形态。因此，这些结果表明，环境而非遗传因素可能是某些极度肥胖人群中出现增生的原因。此外，结果表明脂肪组织基质血管部分中发现的无脂肪细胞具有发育成脂肪细胞的能力。然而，有人认为在这些条件下获得完全分化的脂肪细胞的相对低产率可能是由于最初从中获取这些培养物的原始基质血管部分中脂肪相关细胞的异质性。
