Pierpont G L, DeMaster E G, Cohn J N
Am J Physiol. 1984 Jun;246(6 Pt 2):H824-9. doi: 10.1152/ajpheart.1984.246.6.H824.
Since ischemic heart disease (IHD) is inhomogeneous, the adrenergic response of the heart to ischemia or infarction could depend on the level of adrenergic supply within specific regions of the myocardium. Therefore we quantified myocardial norepinephrine (NE) content of tissue samples from 54 different sites in the left ventricle (LV) of four normal dogs using high-pressure liquid chromatography with electrochemical detection. Up to 10-fold differences in NE content occurred within a single LV. The NE distribution followed a consistent pattern, demonstrating a gradient from apex (avg minimum value of 162 ng/g of tissue) to base (avg maximum value 844 ng/g). No epicardial-to-endocardial gradient was present. In six other dogs a similar pattern was found in myocardial uptake of radioactively labeled NE and epinephrine assessed 2 min after intravenous injection. These results suggest that areas of high tissue NE represent regions rich in adrenergic supply and high in adrenergic activity, not merely NE stored in inactive pools. Samples from a human autopsy specimen suggest that these findings can be extrapolated to humans.
由于缺血性心脏病（IHD）具有不均一性，心脏对缺血或梗死的肾上腺素能反应可能取决于心肌特定区域内的肾上腺素能供应水平。因此，我们使用带电化学检测的高压液相色谱法，对4只正常犬左心室（LV）54个不同部位的组织样本中的心肌去甲肾上腺素（NE）含量进行了量化。在单个左心室内，NE含量存在高达10倍的差异。NE分布遵循一致的模式，显示出从心尖（组织平均最小值为162 ng/g）到心底（平均最大值844 ng/g）的梯度。不存在心外膜到心内膜的梯度。在另外6只犬中，静脉注射2分钟后评估的放射性标记NE和肾上腺素的心肌摄取情况也发现了类似模式。这些结果表明，高组织NE区域代表肾上腺素能供应丰富且肾上腺素能活性高的区域，而不仅仅是储存于非活性池中的NE。来自一份人类尸检标本的样本表明，这些发现可外推至人类。
