辐射和冷冻对小肠结肠炎耶尔森菌[3H]DNA的影响。
Effect of radiation and freezing on [3H]DNA of Yersinia enterocolitica.
作者信息
Grecz N, El-Zawahry Y A
出版信息
Appl Environ Microbiol. 1984 May;47(5):1101-5. doi: 10.1128/aem.47.5.1101-1105.1984.
Abstract
Freezing of the enteropathogenic bacterium Yersinia enterocolitica to -18 and -75 degrees C caused 7 and 42% cell death, respectively, and 0.329 and 0.588 single-strand breaks per 10(8) daltons of DNA, respectively, while radiation to one D10 dose (10% cell survival) combined with freezing to 2 to 0, -18, and -75 degrees C induced 0.05, 0.75, and 5.04 single-strand breaks, respectively. The increase in the effectiveness of radiation with respect to the yield of single-strand breaks at -18 and -75 degrees C is contrary to expectation and seems to be due to arrest of repair of single-strand breaks by these low temperatures.
摘要
将肠道致病菌小肠结肠炎耶尔森氏菌冷冻至-18℃和-75℃分别导致7%和42%的细胞死亡，每10⁸道尔顿DNA分别出现0.329和0.588个单链断裂，而辐射至一个D10剂量（10%细胞存活）并结合冷冻至2至0℃、-18℃和-75℃分别诱导出0.05、0.75和5.04个单链断裂。在-18℃和-75℃下，辐射对单链断裂产生量的有效性增加与预期相反，似乎是由于这些低温导致单链断裂修复受阻。
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