• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

辐射和冷冻对小肠结肠炎耶尔森菌[3H]DNA的影响。

Effect of radiation and freezing on [3H]DNA of Yersinia enterocolitica.

作者信息

Grecz N, El-Zawahry Y A

出版信息

Appl Environ Microbiol. 1984 May;47(5):1101-5. doi: 10.1128/aem.47.5.1101-1105.1984.

DOI:10.1128/aem.47.5.1101-1105.1984
PMID:6742827
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240069/
Abstract

Freezing of the enteropathogenic bacterium Yersinia enterocolitica to -18 and -75 degrees C caused 7 and 42% cell death, respectively, and 0.329 and 0.588 single-strand breaks per 10(8) daltons of DNA, respectively, while radiation to one D10 dose (10% cell survival) combined with freezing to 2 to 0, -18, and -75 degrees C induced 0.05, 0.75, and 5.04 single-strand breaks, respectively. The increase in the effectiveness of radiation with respect to the yield of single-strand breaks at -18 and -75 degrees C is contrary to expectation and seems to be due to arrest of repair of single-strand breaks by these low temperatures.

摘要

将肠道致病菌小肠结肠炎耶尔森氏菌冷冻至-18℃和-75℃分别导致7%和42%的细胞死亡,每10⁸道尔顿DNA分别出现0.329和0.588个单链断裂,而辐射至一个D10剂量(10%细胞存活)并结合冷冻至2至0℃、-18℃和-75℃分别诱导出0.05、0.75和5.04个单链断裂。在-18℃和-75℃下,辐射对单链断裂产生量的有效性增加与预期相反,似乎是由于这些低温导致单链断裂修复受阻。

相似文献

1
Effect of radiation and freezing on [3H]DNA of Yersinia enterocolitica.辐射和冷冻对小肠结肠炎耶尔森菌[3H]DNA的影响。
Appl Environ Microbiol. 1984 May;47(5):1101-5. doi: 10.1128/aem.47.5.1101-1105.1984.
2
Inactivation and injury of Yersinia enterocolitica by radiation and freezing.辐射和冷冻对小肠结肠炎耶尔森菌的灭活及损伤作用
Appl Environ Microbiol. 1981 Sep;42(3):464-8. doi: 10.1128/aem.42.3.464-468.1981.
3
[Formation of double-stranded DNA breaks in gamma-irradiated bacterial cells depending on the functioning of the rapid repair of single-stranded breaks].γ射线辐照细菌细胞中双链DNA断裂的形成取决于单链断裂快速修复的功能
Radiobiologiia. 1981 Jan-Feb;21(1):38-44.
4
Control of Yersinia enterocolitica in raw pork and pork products by gamma-irradiation.通过伽马射线辐照控制生猪肉及猪肉制品中的小肠结肠炎耶尔森菌。
Int J Food Microbiol. 1997 Apr 29;36(1):69-76. doi: 10.1016/s0168-1605(96)01249-4.
5
R.b.e. of 50 k Vp x-rays and 660 keV gamma-rays (137 Cs) with respect to the production of DNA damage, repair and cell-killing in Escherichia coli K-12.关于在大肠杆菌K-12中产生DNA损伤、修复和细胞杀伤方面,50千伏峰值X射线和660千电子伏特γ射线(137铯)的相对生物效应。
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Dec;28(6):539-48. doi: 10.1080/09553007514551401.
6
[Participation of the product of gene dnaG in the slow nonmutagenic repair pathway for gamma-induced single-stranded DNA breaks in Escherichia coli cells].[基因dnaG的产物参与大肠杆菌细胞中γ射线诱导的单链DNA断裂的缓慢非诱变修复途径]
Tsitologiia. 1980 Apr;22(4):454-61.
7
Dependence of the yield of strand breaks induced by gamma-rays in DNA on the physical conditions of exposure: water content and temperature.DNA中γ射线诱导的链断裂产率对辐照物理条件(含水量和温度)的依赖性。
Int J Radiat Biol. 1993 Mar;63(3):289-96. doi: 10.1080/09553009314550391.
8
Letter: Rejoining of radiation-induced single-strand breaks in DNA of Escherichia coli: involvement of RNA synthesis.
Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Oct;28(4):385-9. doi: 10.1080/09553007514551161.
9
Induction and repair of DNA strand breaks and 1-beta-D-arabinofuranosylcytosine-detectable sites in 40-75 kVp X-irradiated compared to 60Co gamma-irradiated human cell lines.与60Coγ射线辐照的人类细胞系相比,40 - 75 kVp X射线辐照下DNA链断裂和1-β-D-阿拉伯呋喃糖基胞嘧啶可检测位点的诱导与修复
Radiat Res. 1988 Apr;114(1):168-85.
10
[Effect of cysteine on single-stranded breaks induced by ionizing radiation and on their repair].[半胱氨酸对电离辐射诱导的单链断裂及其修复的影响]
Radiobiologiia. 1975 Nov-Dec;15(6):913-6.

引用本文的文献

1
Freezing shortens the lifetime of DNA molecules under tension.冷冻会缩短处于张力状态下的DNA分子的寿命。
J Biol Phys. 2017 Dec;43(4):511-524. doi: 10.1007/s10867-017-9466-3. Epub 2017 Sep 8.
2
Reduced virulence of Yersinia enterocolitica by copper-induced injury.铜诱导损伤降低小肠结肠炎耶尔森菌的毒力
Appl Environ Microbiol. 1985 Aug;50(2):406-11. doi: 10.1128/aem.50.2.406-411.1985.
3
In situ freezing of the rat urinary bladder: DNA adduct formation in the bladder epithelium demonstrated by 32P-postlabeling assay.大鼠膀胱原位冷冻:通过³²P后标记法检测膀胱上皮细胞中的DNA加合物形成。
Jpn J Cancer Res. 1989 Sep;80(9):826-32. doi: 10.1111/j.1349-7006.1989.tb01722.x.

本文引用的文献

1
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
Inactivation and injury of Yersinia enterocolitica by radiation and freezing.辐射和冷冻对小肠结肠炎耶尔森菌的灭活及损伤作用
Appl Environ Microbiol. 1981 Sep;42(3):464-8. doi: 10.1128/aem.42.3.464-468.1981.
3
Radiation treatment of foods. I. Radurization of fresh eviscerated poultry.食品的辐射处理。I. 新鲜去内脏家禽的辐射巴氏杀菌
Appl Microbiol. 1968 Jul;16(7):1061-6. doi: 10.1128/am.16.7.1061-1066.1968.
4
X-ray-induced single-strand breaks in DNA of E. coli B-r: effect of irradiation and postirradiation conditions.
Radiat Res. 1971 Feb;45(2):299-310.
5
Production and repair of radiochemical damage in Escherichia coli deoxyribonucleic acid; its modification by culture conditions and relation to survival.大肠杆菌脱氧核糖核酸中放射化学损伤的产生与修复;其受培养条件的影响及与存活的关系
J Bacteriol. 1971 Jan;105(1):127-35. doi: 10.1128/jb.105.1.127-135.1971.
6
Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.受辐照大肠杆菌脱氧核糖核酸的体内重建;断裂片段的重新连接。
Nature. 1966 Oct 29;212(5061):534-5. doi: 10.1038/212534a0.
7
DNA-strand scission and loss of viability after x irradiation of normal and sensitized bacterial cells.正常细菌细胞和致敏细菌细胞经X射线照射后的DNA链断裂及生存力丧失
Proc Natl Acad Sci U S A. 1966 Jun;55(6):1442-6. doi: 10.1073/pnas.55.6.1442.
8
Resistance of DNA against radiation-induced strand breakage in bacterial spores.细菌芽孢中DNA对辐射诱导链断裂的抗性。
Radiat Res. 1970 Sep;43(3):613-26.
9
Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M.大肠杆菌噬菌体及大肠杆菌噬菌体DNA的分子量。IV. 来自噬菌体T4、T5和T7的DNA的分子量以及分子量测定的一般问题
J Mol Biol. 1970 Dec 28;54(3):567-77. doi: 10.1016/0022-2836(70)90127-0.
10
DNA strand breakage in cells irradiated with x-rays.受X射线照射的细胞中的DNA链断裂。
Nature. 1969 Jun 14;222(5198):1042-4. doi: 10.1038/2221042a0.