Packer L, Arrio B, Johannin G, Volfin P
Biochem Biophys Res Commun. 1984 Jul 18;122(1):252-8. doi: 10.1016/0006-291x(84)90467-4.
Laser Doppler velocimetry measurements on purple membrane suspensions from Halobacterium halobium showed a linear correlation between electrophoretic mobility and applied electric field, electrokinetic responses could be rapidly monitored. Native membranes are less charged than white membrane preparations (from the R1mW mutant). Chemical modification of carboxyl residues reduces surface charge, and nitrotyrosine modified membranes are more or less charged than native membranes at pH greater than or less than 6.5, respectively. Changes in surface charge are found upon actinic illumination and are greatest (Ca 5 X 10(-4)/A2) under conditions where decay of the M412 intermediate of the photoreaction cycle is inhibited, such as at high pH or after chemical modification.
对嗜盐菌紫膜悬浮液进行激光多普勒测速测量表明，电泳迁移率与外加电场之间呈线性相关，电动响应能够被快速监测。天然膜的电荷比白色膜制剂（来自R1mW突变体）少。羧基残基的化学修饰会降低表面电荷，而硝基酪氨酸修饰的膜在pH大于或小于6.5时，分别比天然膜带更多或更少的电荷。在光化照射后会发现表面电荷的变化，并且在光反应循环的M412中间体衰变受到抑制的条件下（如在高pH值或化学修饰后）变化最大（约5×10⁻⁴/A²）。
