Thelen M, Petrone G, O'Shea P S, Azzi A
Biochim Biophys Acta. 1984 Jul 27;766(1):161-8. doi: 10.1016/0005-2728(84)90228-7.
The synthesis and characterisation of fluorescein-phosphatidylethanolamine (FPE) is described. The effects of dielectric constant, ionic strength and ambient pH upon the optical absorbance properties of FPE are presented. It is shown that under appropriate conditions, FPE rapidly and quantitatively reports the pH of the aqueous bulk phases when incorporated into phospholipid vesicles. It is also shown that, when the external medium is highly buffered, FPE is capable of specificity reporting only the pH of the intravesicular compartment. The application of FPE for studies of intravesicular pH changes of reconstituted membranous protein systems is discussed.
本文描述了荧光素 - 磷脂酰乙醇胺（FPE）的合成与表征。介绍了介电常数、离子强度和环境pH对FPE光吸收特性的影响。结果表明，在适当条件下，当FPE掺入磷脂囊泡时，它能快速、定量地反映水相主体的pH值。还表明，当外部介质具有高缓冲能力时，FPE仅能特异性地反映囊泡内隔室的pH值。讨论了FPE在重组膜蛋白系统囊泡内pH变化研究中的应用。
