Soucaille P, Prats M, Tocanne J F, Teissié J
Centre de recherches de Biochimie et de Génétique cellulaires du CNRS, Toulouse, France.
Biochim Biophys Acta. 1988 Apr 7;939(2):289-94. doi: 10.1016/0005-2736(88)90073-9.
A fluorescent indicator for the determination of pH in the vicinity of water/lipid interfaces was produced by the covalent linkage of fluoresceinisothiocyanate to Escherichia coli phosphatidylethanolamine. When embedded in monolayers spread on an air/water interface, its apparent pK was shown to be only slightly affected by the nature of the polar headgroups or the packing density of the host phospholipids. Its properties were not affected by the ion content of the subphase. For small unilamellar vesicles, its properties were only affected when localized in the inner layer. This probe could therefore be of value in the study of proton fluxes along biological membranes.
通过将异硫氰酸荧光素与大肠杆菌磷脂酰乙醇胺共价连接，制备了一种用于测定水/脂质界面附近pH值的荧光指示剂。当包埋在铺展于空气/水界面的单分子层中时，其表观pK值仅受到极性头部基团性质或主体磷脂堆积密度的轻微影响。其性质不受亚相离子含量的影响。对于小单层囊泡，只有当它位于内层时其性质才会受到影响。因此，该探针在研究沿生物膜的质子通量方面可能具有价值。
