Torre L A, Pérez-Albarsanz M A
Comp Biochem Physiol B. 1984;78(1):299-302. doi: 10.1016/0305-0491(84)90185-8.
To obtain information about androgenic hormone actions on the synthesis of lipids from [1-14C]acetate in rat ventral prostate, in vitro and in vivo experiments have been carried out. The time course of incorporation of labelled acetate was studied. Also different solubilization media of androgenic hormones was investigated. We have selected Tween-80. Results for in vitro experiments, show that increasing concentrations of testosterone and dihydro-testosterone correspond to an increase in the incorporation of labelled acetate into phospholipids and a decrease in the incorporation into triacylglycerols. In vivo experiments of incorporation of [1-14C]acetate have also been carried out and show a similar pattern to the situation found in our in vitro experiments.
为了获取雄激素对大鼠腹侧前列腺中[1-14C]乙酸酯脂质合成作用的信息，已进行了体外和体内实验。研究了标记乙酸酯掺入的时间进程。还研究了雄激素的不同增溶介质。我们选择了吐温80。体外实验结果表明，睾酮和二氢睾酮浓度的增加对应于标记乙酸酯掺入磷脂的增加以及掺入三酰甘油的减少。[1-14C]乙酸酯的体内掺入实验也已进行，其结果与我们体外实验中的情况相似。
