Porschen R, Robin U, Schumacher A, Schauseil S, Borchard F, Hengels K J, Strohmeyer G
Department of Gastroenterology, Heinrich-Heine-University, Dusseldorf, Germany.
Gut. 1992 May;33(5):663-7. doi: 10.1136/gut.33.5.663.
DNA ploidy and S-phase fractions were assessed by flow cytometry in colonic biopsy specimens from 28 patients with ulcerative colitis and 51 with Crohn's disease. Whereas only diploid DNA histograms were found in Crohn's disease and control subjects, three patients with ulcerative colitis exhibited DNA aneuploidy. In one case, aneuploidy was associated with low grade dysplasia. S-phase fractions were higher in ulcerative colitis (mean (SD) 17.8 (7.7)%) than in Crohn's disease (13.1 (4.6)%) or control subjects (14.2 (4.6)%), but did not correlate with either disease activity or duration in any group. In this study, aneuploidy was associated exclusively with ulcerative colitis, even in the absence of dysplasia. In view of the epidemiological differences in malignant colonic transformation between ulcerative colitis and Crohn's disease, this study suggests that flow cytometry may help to identify individuals with an increased cancer risk in ulcerative colitis.
通过流式细胞术对28例溃疡性结肠炎患者和51例克罗恩病患者的结肠活检标本进行DNA倍体和S期分数评估。在克罗恩病患者和对照受试者中仅发现二倍体DNA直方图，而3例溃疡性结肠炎患者表现出DNA非整倍体。在1例中，非整倍体与低度发育异常相关。溃疡性结肠炎患者的S期分数（均值（标准差）17.8（7.7）%）高于克罗恩病患者（13.1（4.6）%）或对照受试者（14.2（4.6）%），但在任何组中均与疾病活动度或病程无关。在本研究中，非整倍体仅与溃疡性结肠炎相关，即使在无发育异常的情况下也是如此。鉴于溃疡性结肠炎和克罗恩病在结肠恶性转化方面的流行病学差异，本研究提示流式细胞术可能有助于识别溃疡性结肠炎中癌症风险增加的个体。
