Arlian L G, Geis D P, Vyszenski-Moher D L, Bernstein I L, Gallagher J S
J Allergy Clin Immunol. 1984 Aug;74(2):166-71. doi: 10.1016/0091-6749(84)90281-1.
The antigens and allergens in the storage mite Tyrophagus putrescentiae, commonly found in grain and hay and sometimes in house dust, were characterized by crossed immunoelectrophoresis and crossed radioimmunoelectrophoresis. Three mite extracts, T. putrescentiae (TP) mite bodies, feces, and a combination of mites, feces, and culture medium (TP + CM) were studied. TP and TP + CM (primarily feces) extracts exhibited 20 and 18 antigens, respectively. By use of serum from two skin test-positive patients, autoradiograms demonstrated TP contained two allergens and TP + CM contained five allergens, three of which probably originated from feces. CM (whole wheat flour) did not stimulate antibody production in rabbits. TP feces shared 10 antigenic determinants with TP and 14 with TP + CM. Two antigens common to TP feces and TP were also shared allergens.
腐食酪螨是一种常见于谷物、干草中，有时也存在于室内灰尘中的仓储螨，其抗原和过敏原通过交叉免疫电泳和交叉放射免疫电泳进行了表征。研究了三种螨提取物，即腐食酪螨（TP）螨体、粪便以及螨、粪便和培养基的组合（TP + CM）。TP提取物和TP + CM提取物（主要是粪便）分别显示出20种和18种抗原。利用两名皮肤试验呈阳性患者的血清，放射自显影片显示TP含有两种过敏原，TP + CM含有五种过敏原，其中三种可能源自粪便。CM（全麦面粉）不会刺激兔子产生抗体。TP粪便与TP共有10个抗原决定簇，与TP + CM共有14个抗原决定簇。TP粪便和TP共有的两种抗原也是共同过敏原。
