Arlian L G, Vyszenski-Moher D L, Johansson S G, van Hage-Hamsten M
Department of Biological Sciences, Wright State University, Dayton, Ohio, USA.
Ann Allergy Asthma Immunol. 1997 Dec;79(6):525-9. doi: 10.1016/S1081-1206(10)63060-8.
Allergic sensitivity to storage mites is common in farmers and others who work in environments where hay and grain are handled, stored, or processed. The clinical importance of storage mite-induced allergy in these individuals is generally unknown in the United States. Tyrophagus putrescentiae is a common storage mite, the allergenicity of which has not been characterized.
The purpose of this study was to characterize the allergenicity of Tyrophagus putrescentiae.
Crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE) were performed to characterize the allergens of this mite using the sera from 24 occupationally exposed farmers RAST-positive to T. putrescentiae.
CRIE analysis of T. putrescentiae extract utilizing individual farmers' sera identified 14 allergens. The number of allergens that bound IgE in the individual sera ranged from 5 to 11. Sensitivity to the different allergens varied both within and between patients but 100% of the farmers' sera recognized the same five allergens precipitated by CIE. Fifty percent of patients' sera recognized one additional. Forty-two and 21% of the sera had IgE binding to six and seven of the allergens, respectively.
The results of this study showed that farmers who were occupationally exposed to storage mites had serum IgE specific for many potent allergens from T. putrescentiae. Persons who are exposed to stored product mites in occupational settings or by consumption of food containing these mites are therefore at risk of sensitization and allergic reaction. Increased awareness of sensitivity to this mite in the United States' population is needed.
对仓储螨的过敏敏感性在农民以及其他从事干草和谷物处理、储存或加工环境工作的人群中很常见。在美国，这些个体中由仓储螨引起的过敏的临床重要性通常尚不清楚。腐食酪螨是一种常见的仓储螨，其致敏性尚未得到表征。
本研究的目的是表征腐食酪螨的致敏性。
使用24名职业性接触腐食酪螨且RAST检测呈阳性的农民的血清，进行交叉免疫电泳（CIE）和交叉放射免疫电泳（CRIE），以表征该螨的过敏原。
利用个体农民血清对腐食酪螨提取物进行CRIE分析，鉴定出14种过敏原。个体血清中与IgE结合的过敏原数量在5至11种之间。患者内部和患者之间对不同过敏原的敏感性各不相同，但100%的农民血清识别出CIE沉淀的相同5种过敏原。50%的患者血清还识别出另外一种过敏原。分别有42%和21%的血清IgE与6种和7种过敏原结合。
本研究结果表明，职业性接触仓储螨的农民血清IgE对腐食酪螨的许多强效过敏原具有特异性。因此，在职业环境中接触储存产品螨或通过食用含有这些螨的食物的人有致敏和过敏反应的风险。美国人群需要提高对这种螨敏感性的认识。
