噬菌体P22作为鼠伤寒沙门氏菌中Mu诱变的载体：nad-lac和pnc-lac基因融合体的分离
Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.
作者信息
Holley E A, Foster J W
出版信息
J Bacteriol. 1982 Nov;152(2):959-62. doi: 10.1128/jb.152.2.959-962.1982.
Abstract
Salmonella phage P22 was utilized as a vector for phage Mu cts d1(Apr lac) mutagenesis in Salmonella typhimurium. Efficient transposition of phage Mu d1 and the construction of gene fusions were readily accomplished with this procedure. Mutants blocked in the biosynthesis of NAD+ and in pyridine nucleotide cycle metabolism were isolated by this method, resulting in nadB-lac, nadC-lac, and pncB-lac gene fusions.
摘要
沙门氏菌噬菌体P22被用作在鼠伤寒沙门氏菌中进行噬菌体Mu cts d1（Apr lac）诱变的载体。通过该程序可轻松实现噬菌体Mu d1的高效转座和基因融合的构建。用此方法分离出了在NAD +生物合成及吡啶核苷酸循环代谢中受阻的突变体，从而得到了nadB - lac、nadC - lac和pncB - lac基因融合体。
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