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在含有质粒pKM101的鼠伤寒沙门氏菌LT2中紫外线照射的噬菌体P22的诱导再激活和诱变

Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Salmonella typhimurium LT2 containing the plasmid pKM101.

作者信息

Walker G C

出版信息

J Bacteriol. 1978 Aug;135(2):415-21. doi: 10.1128/jb.135.2.415-421.1978.

Abstract

The inducible (Weigle) reactivation of UV-irradiated bacteriophage P22 has been examined on strains of Salmonella typhimurium with and without the mutagenesis-enhancing plasmid pKM101. A large inducible reactivation was observed in the plasmid-containing strain, but only a small response was observed in the strain lacking the plasmid. An increased frequency of clear-plaque mutants was detected among the survivors. The efficiencies of the plasmid-mediated and cellular repair processes have been determined. The kinetics of induction of the phage reactivation have been investigated. The relationship of the observed results to the inducible reactivation of UV-irradiated lambda in Escherichia coli and to error-prone repair is discussed.

摘要

在含有和不含有诱变增强质粒pKM101的鼠伤寒沙门氏菌菌株上,检测了紫外线照射的噬菌体P22的诱导性(韦格尔)复活情况。在含质粒的菌株中观察到大量的诱导性复活,但在缺乏质粒的菌株中仅观察到小的反应。在存活者中检测到空斑突变体的频率增加。已确定了质粒介导的修复过程和细胞修复过程的效率。研究了噬菌体复活诱导的动力学。讨论了观察结果与紫外线照射的λ噬菌体在大肠杆菌中的诱导性复活以及易错修复的关系。

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The muc genes of pKM101 are induced by DNA damage.pKM101的muc基因由DNA损伤诱导。
J Bacteriol. 1983 Sep;155(3):1306-15. doi: 10.1128/jb.155.3.1306-1315.1983.

本文引用的文献

8
Recovery of phage lambda from ultraviolet damage.从紫外线损伤中恢复噬菌体λ。
Basic Life Sci. 1975;5A:155-71. doi: 10.1007/978-1-4684-2895-7_20.

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