天然二倍体白色念珠菌的重组分析
Recombination analysis of naturally diploid Candida albicans.
作者信息
Poulter R, Hanrahan V, Jeffery K, Markie D, Shepherd M G, Sullivan P A
出版信息
J Bacteriol. 1982 Dec;152(3):969-75. doi: 10.1128/jb.152.3.969-975.1982.
A multiply auxotrophic strain, hOG45, was derived from Candida albicans ATCC 10261. Prototrophic revertants of this multiple auxotroph were selected after mutagenesis. These prototrophic revertants were distinguishable from the original prototroph, ATCC 10261, because of their mitotic instability. They gave rise to auxotrophic derivatives which displayed one or more of the auxotrophic requirements characteristic of hOG45. Two of the auxotrophic requirements, those for adenine and methionine, frequently reappeared together in the auxotrophic derivatives of the prototrophic revertants. This apparent linkage of ade and met was confirmed by protoplast fusion analysis of the original auxotroph. These data indicate that C. albicans ATCC 10261 is diploid, the multiple auxotroph h0G45 is homozygous for recessive auxotrophic alleles, the prototrophic revertants are multiple heterozygotes, the auxotrophic derivatives are homozygotes produced by mitotic crossing-over, and the association between the ade and met alleles is due to linkage.
一种多重营养缺陷型菌株hOG45源自白色念珠菌ATCC 10261。该多重营养缺陷型菌株经诱变后筛选出原养型回复突变体。这些原养型回复突变体因其有丝分裂不稳定性而与原始原养型菌株ATCC 10261不同。它们产生了营养缺陷型衍生物，这些衍生物表现出hOG45所特有的一种或多种营养缺陷需求。其中两种营养缺陷需求，即对腺嘌呤和蛋氨酸的需求，经常在原养型回复突变体的营养缺陷型衍生物中同时再次出现。通过对原始营养缺陷型菌株的原生质体融合分析，证实了ade和met的这种明显连锁关系。这些数据表明，白色念珠菌ATCC 10261是二倍体，多重营养缺陷型菌株h0G45对隐性营养缺陷等位基因是纯合的，原养型回复突变体是多重杂合子，营养缺陷型衍生物是通过有丝分裂交换产生的纯合子，ade和met等位基因之间的关联是由于连锁。
Zotero 插件