Farris S H, Sahota T S, Ibaraki A, Thomson A J
Stain Technol. 1982 Sep;57(5):283-8. doi: 10.3109/10520298209066724.
Scale tissues taken from cones of pendent Douglas-fir Pseudotsuga menziesii (Mirb.) Franco were treated with pectinase to dissociate nuclei for Feulgen staining. Hydration of fixed tissues with water or a graded series of ethyl alcohol before pectinase treatment resulted in distention and faint staining of nuclei and inadequate disintegration of cell walls. These problems were overcome by adopting citrate buffer hydration. Analysis of digitized scans of nuclei provided information about the distribution of the stained material in density classes and nuclear size which is usually unavailable through the conventional double wavelength DNA measuring method.
取自下垂花旗松（Pseudotsuga menziesii (Mirb.) Franco）球果的鳞片组织用果胶酶处理以解离细胞核用于孚尔根染色。在果胶酶处理前用水或一系列分级乙醇对固定组织进行水化处理，导致细胞核膨胀、染色浅且细胞壁解体不充分。通过采用柠檬酸盐缓冲液水化克服了这些问题。对细胞核数字化扫描的分析提供了有关染色物质在密度类别和核大小方面分布的信息，而这些信息通常是通过传统的双波长DNA测量方法无法获得的。
