酵母苯丙氨酸转运核糖核酸中A9与AU12之间三重相互作用的核磁共振观察。
Nuclear magnetic resonance observation of the triple interaction between A9 and AU12 in yeast tRNAPhe.
作者信息
Choi B S, Redfield A G
出版信息
Nucleic Acids Res. 1985 Jul 25;13(14):5249-54. doi: 10.1093/nar/13.14.5249.
Abstract
The nuclear Overhauser effect (NOE) was used to identify one of the amino proton resonances of base A23 in interaction with A9. These bases form a triple with U12 in the D stem of yeast tRNAPhe. The identification was verified by finding an NOE from this amino proton to the C8 proton of A9, as determined by comparisons of NOE's in a native and a C8-deuterated sample.
摘要
利用核Overhauser效应（NOE）来识别与A9相互作用的碱基A23的一个氨基质子共振峰。这些碱基在酵母苯丙氨酸转运RNA（tRNAPhe）的D茎中与U12形成三联体。通过在天然样品和C8氘代样品中比较NOE，发现从该氨基质子到A9的C8质子存在NOE，从而验证了该识别。
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