Mitrani E, Marks R
Arch Dermatol Res. 1982;274(1-2):21-8. doi: 10.1007/BF00510354.
The distribution of procollagen in normal hyperplastic, preneoplastic or neoplastic human epidermal lesions has been analysed in indirect immunofluorescence tests with the antibodies to procollagen raised in sheep to extracted procollagen, synthesised by newborn rat skin explants in culture. These antiprocollagen antibodies produced indirect immunofluorescence staining only of the papillary dermis of human skin. We reacted serial dilutions of the antibody with each skin lesion and recorded the maximum dilution at which a positive reaction was observed. All lesions examined displayed essentially the same procollagen immunofluorescence pattern. The fluorescence was localised as a fibrillar/diffuse area just under the epidermis. In conditions in which the epidermis is highly convoluted, the fluorescent band was found to follow the pattern of the epidermis and to surround epidermal 'islands' in the deeper dermis. Our observations suggest that in the neoplastic lesions a new dermal topography, resembling the stratum papillare of normal human skin, is found in the deeper dermis surrounding the epidermal islands produced as the epidermal mass increases and invaginates further into the stroma. Malignant epidermal lesions were found to show a positive procollagen immunofluorescent staining at tenfold lower concentrations of the antibody than normal human skin in subepidermal regions.
采用针对新生大鼠皮肤外植体在培养中合成并提取的前胶原，由绵羊制备的抗前胶原抗体，通过间接免疫荧光试验分析了正常人增生性、癌前或肿瘤性人类表皮病变中前胶原的分布情况。这些抗前胶原抗体仅对人类皮肤的乳头真皮产生间接免疫荧光染色。我们将抗体的系列稀释液与每个皮肤病变进行反应，并记录观察到阳性反应的最大稀释度。所有检查的病变显示出基本相同的前胶原免疫荧光模式。荧光定位在表皮下方的一个纤维状/弥漫性区域。在表皮高度卷曲的情况下，发现荧光带遵循表皮的模式，并在更深的真皮中围绕表皮“岛”。我们的观察结果表明，在肿瘤性病变中，随着表皮团块增加并进一步向基质内陷，在围绕产生的表皮岛的更深真皮中发现了一种类似于正常人皮肤乳头层的新真皮地形。发现恶性表皮病变在表皮下区域显示出阳性前胶原免疫荧光染色时，所需抗体浓度比正常人皮肤低十倍。
