Weber L, Krieg T, Müller P K, Kirsch E, Timpl R
Br J Dermatol. 1982 Mar;106(3):267-73. doi: 10.1111/j.1365-2133.1982.tb01722.x.
Purified antibodies against type IV collagen and laminin were used ot localize basement membranes by indirect immunofluorescence in various anatomical regions of normal and diseased human skin. The two proteins showed extensive codistribution. A continuous linear staining was found along the epidermal-dermal junction and around hair follicles, sebaceous gland acini and small capillaries. The same proteins also surrounded individual cells such as those found in vessels, hair erector muscles and subcutaneous tissue. Blister formation in bullous pemphigoi left type IV collagen and laminin on the floor of the blister, while the bullous pemphigoid antigen as detected by human autoantibodies was found on both sides of the blister. In solid basal cell carcinoma a strong staining was found around all tumour islands as well as focally within the cell clusters. This suggests that the tumour cells produce these basement membrane proteins but have lost, at least in part, control of polar deposition.
抗IV型胶原蛋白和层粘连蛋白的纯化抗体通过间接免疫荧光法用于在正常和患病人类皮肤的各个解剖区域定位基底膜。这两种蛋白质显示出广泛的共分布。在表皮-真皮交界处以及毛囊、皮脂腺腺泡和小毛细血管周围发现连续的线性染色。相同的蛋白质也围绕着单个细胞，如血管、立毛肌和皮下组织中的细胞。大疱性类天疱疮中的水疱形成使水疱底部留下IV型胶原蛋白和层粘连蛋白，而人自身抗体检测到的大疱性类天疱疮抗原则在水疱两侧发现。在实体性基底细胞癌中，在所有肿瘤岛周围以及细胞簇内局部发现强染色。这表明肿瘤细胞产生这些基底膜蛋白，但至少部分失去了对极性沉积的控制。
