Varigos G A, Morstyn G, Vadas M A
Clin Exp Immunol. 1982 Dec;50(3):555-62.
An eosinophil stimulating material (ESM) is described in the blister fluids (BF) of six patients with bullous pemphigoid (BP). This ESM is similar in function to a subspecies of human colony stimulating factor (CSF), CSF-alpha, since (i) it stimulated the production from bone marrow cells of day 14 colonies (31 +/- 15, arithmetic mean +/- 1 s.e.m. colonies, n = 5) in semi-solid agar, (ii) a large proportion (47 +/- 12%, n = 5) of these colonies were eosinophilic and (iii) it activated purified peripheral eosinophils as judged by increased autofluorescence (a mean increase of 23.6 +/- 6.6 units measured by flow cytometry, n = 10). BF from patients with four other blistering diseases, burn blisters, suction blisters from normal, eosinophilic or BP patients, various sera, histamine and eosinophil chemotactic factor (ECF-A) lacked activities in these assays. This description of a tissue accumulation of a CSF like substance suggests that there exists a physiological role for CSF in the periphery. In addition it supports the concept that local activation of eosinophils contributes to the pathogenesis of BP.
在6例大疱性类天疱疮（BP）患者的水疱液（BF）中发现了一种嗜酸性粒细胞刺激物质（ESM）。这种ESM在功能上与人类集落刺激因子（CSF）的一个亚类CSF-α相似，因为：（i）它能刺激半固体琼脂中骨髓细胞产生第14天的集落（31±15，算术平均值±1个标准误集落，n = 5）；（ii）这些集落中有很大比例（47±12%，n = 5）是嗜酸性的；（iii）通过自发荧光增加判断（通过流式细胞术测量平均增加23.6±6.6个单位，n = 10），它能激活纯化的外周嗜酸性粒细胞。来自其他四种水疱性疾病患者的BF、烧伤水疱、正常、嗜酸性或BP患者的抽吸水疱、各种血清、组胺和嗜酸性粒细胞趋化因子（ECF-A）在这些试验中均无活性。这种CSF样物质在组织中积聚的描述表明外周存在CSF的生理作用。此外，它支持嗜酸性粒细胞的局部激活参与BP发病机制的概念。
