Chan A C, Allen C E, Hegarty P V
J Nutr. 1980 Jan;110(1):66-73. doi: 10.1093/jn/110.1.66.
The effects of dietary vitamin E-depletion and repletion on the cyclooxygenase activity was studied in the semitendinosus muscle of rabbits. The prostaglandin (PG) cyclooxygenase system in rabbit semitendinosus muscle was characterized and found to depend on reduced glutathione and 1-epinephrine as cofactors. Skeletal muscle cyclooxygenase generates PGE2 and PGF2 alpha at a ratio approximately equal to one. Weanling New Zealand white rabbits were fed a vitamin E-deficient diet for 4 to 5 weeks. Controls received 50 mg dl-alpha-tocopherol acetate twice weekly. Vitamin E deficiency caused a significant reduction in cyclooxygenase activity but did not change the PGE2/PGF2 alpha ratio. Oral supplementation of tocopherol acetate promptly returned the cyclooxygenase activity back to the control values within 48 hours. The decreased cyclooxygenase activity explains in part the increased level of arachidonic acid in skeletal muscle phospholipid previously reported in this laboratory. The possible involvement of decreased prostaglandin endoperoxides with platelet aggregation in vitamin E deficiency is discussed.
研究了膳食中维生素E缺乏和补充对兔半腱肌中环氧化酶活性的影响。对兔半腱肌中的前列腺素（PG）环氧化酶系统进行了表征，发现其依赖于还原型谷胱甘肽和1-肾上腺素作为辅因子。骨骼肌环氧化酶产生PGE2和PGF2α的比例约为1:1。将断奶的新西兰白兔喂食维生素E缺乏饮食4至5周。对照组每周两次接受50毫克dl-α-生育酚醋酸酯。维生素E缺乏导致环氧化酶活性显著降低，但未改变PGE2/PGF2α比例。口服补充生育酚醋酸酯可在48小时内迅速使环氧化酶活性恢复到对照值。环氧化酶活性降低部分解释了本实验室先前报道的骨骼肌磷脂中花生四烯酸水平升高的现象。讨论了维生素E缺乏时前列腺素内过氧化物减少与血小板聚集的可能关系。
