Marshak-Rothstein A, Siekevitz M, Margolies M N, Mudgett-Hunter M, Gefter M L
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1120-4. doi: 10.1073/pnas.77.2.1120.
Hybridoma cell lines that secrete monoclonal antiazophenylarsonate antibodies were isolated from the fusion of A/J splenic lymphocytes with a myeloma cell line. A small percentage of these hybridoma proteins were recognized by rabbit antisera that detect the crossreactive idiotype characteristics of the antiazophenylarsonate response of A/J mice. The isotype, pI value, and amino-terminal sequences of four independently derived idiotype-positive hybridoma proteins were determined. These proteins were either of the IgG1 or IgG2a heavy chain class. For two mice tested, the majority of the idiotype in the immune serum was shown to be of the same isotype as the fusion-derived monoclonal antibodies. The pI values of the hybridoma proteins differed from one another and ranged from 6.9 to 7.6. Amino acid sequences of the heavy chains showed a significant degree of homology with each other, but each chain was unique in the framework or the first complementarity determining region (or both). A comparable pattern of sequence variation was evident for the light chains. The azophenylarsonate idiotype, therefore, appears to consist of a family of nonidentical but closely related molecules that are the product of more than one germline gene or the result of somatic mutation of a single germline gene.
通过将A/J脾淋巴细胞与骨髓瘤细胞系融合，分离出了分泌抗偶氮苯胂酸盐单克隆抗体的杂交瘤细胞系。这些杂交瘤蛋白中有一小部分能被兔抗血清识别，该抗血清可检测A/J小鼠抗偶氮苯胂酸盐反应的交叉反应独特型特征。测定了四种独立衍生的独特型阳性杂交瘤蛋白的同种型、pI值和氨基末端序列。这些蛋白要么属于IgG1重链类，要么属于IgG2a重链类。对于所检测的两只小鼠，免疫血清中的大多数独特型与融合衍生的单克隆抗体具有相同的同种型。杂交瘤蛋白的pI值彼此不同，范围在6.9至7.6之间。重链的氨基酸序列彼此显示出显著程度的同源性，但每条链在框架区或第一个互补决定区（或两者）都是独特的。轻链也有类似的序列变异模式。因此，偶氮苯胂酸盐独特型似乎由一族不相同但密切相关的分子组成，这些分子是多个种系基因的产物，或是单个种系基因体细胞突变的结果。
