Winter J U, Wolfe R S
Arch Microbiol. 1980 Jan;124(1):73-9. doi: 10.1007/BF00407031.
When Acetobacterium woodii was co-cultured in continuous or in stationary culture with Methanobacterium strain AZ, fructose instead of being converted to 3 mol of acetate was converted to 2 mol of acetate and 1 mol each of carbon dioxide and methane, showing that interspecies hydrogen transfer occurred. In continuous culture the organisms formed a close physical association in clumps; the doubling time for each organism was 6 h at 33 degrees C. Methane mainly was derived from carbon positions 3 and 4 of the sugar, but other carbons also yielded methane; this was shown to be due to carbon dioxide-acetate exchange reactions by A. woodii in a manner similar to that carried out by Clostridium thermoaceticum. Four other methanogens, Methanobacterium M.o.H. and M.o.H. G, Methanobacterium formicicum, and Methanosarcina barkeri (not acetate-adapted) also produced similar results, when co-cultured with A. woodii.
当伍氏醋酸杆菌与AZ甲烷杆菌菌株在连续培养或静置培养中共同培养时，果糖未被转化为3摩尔乙酸盐，而是被转化为2摩尔乙酸盐以及各1摩尔的二氧化碳和甲烷，这表明种间氢转移发生了。在连续培养中，这些微生物紧密聚集形成物理关联；在33摄氏度时，每种微生物的倍增时间为6小时。甲烷主要来源于糖的3号和4号碳位，但其他碳位也能产生甲烷；这表明伍氏醋酸杆菌以类似于热醋酸梭菌的方式进行二氧化碳-乙酸盐交换反应。当与伍氏醋酸杆菌共同培养时，其他四种产甲烷菌，即甲烷杆菌M.o.H.和M.o.H. G、甲酸甲烷杆菌以及巴氏甲烷八叠球菌（未适应乙酸盐）也产生了类似的结果。
