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通过高压液相色谱法对各种生物流体中结合胆红素和未结合胆红素IX-α进行准确且灵敏的分析。

An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-alpha in various biological fluids.

作者信息

Onishi S, Itoh S, Kawade N, Isobe K, Sugiyama S

出版信息

Biochem J. 1980 Jan 1;185(1):281-4. doi: 10.1042/bj1850281.

DOI:10.1042/bj1850281
PMID:6769432
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1161298/
Abstract

An accurate and sensitive method was developed for the complete separation of the native tetrapyrroles, such as bilirubin and its mono- and di-conjugates of glucuronic acid, glucose and xylose, by ion-pair reversed-phase high-pressure liquid chromatography. The application of this method was demonstrated by the analysis of bile pigments in human bile and urine, and the method also makes it possible to estimate very low UDP-glucuronyltransferase activity, such as is found in the human foetal and neonatal liver.

摘要

通过离子对反相高压液相色谱法，开发了一种准确且灵敏的方法，用于完全分离天然四吡咯，如胆红素及其葡萄糖醛酸、葡萄糖和木糖的单共轭物和双共轭物。通过分析人胆汁和尿液中的胆汁色素证明了该方法的应用，并且该方法还能够估计极低的尿苷二磷酸葡萄糖醛酸基转移酶活性，如在人胎儿和新生儿肝脏中发现的那样。

相似文献

1
An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-alpha in various biological fluids.通过高压液相色谱法对各种生物流体中结合胆红素和未结合胆红素IX-α进行准确且灵敏的分析。
Biochem J. 1980 Jan 1;185(1):281-4. doi: 10.1042/bj1850281.
2
Separation and characterization of bilirubin conjugates by high-performance liquid chromatography.通过高效液相色谱法分离和鉴定胆红素共轭物
J Chromatogr. 1980 Apr 11;182(1):100-4. doi: 10.1016/s0378-4347(00)81656-4.
3
Reverse-phase h.p.l.c. separation, quantification and preparation of bilirubin and its conjugates from native bile. Quantitative analysis of the intact tetrapyrroles based on h.p.l.c. of their ethyl anthranilate azo derivatives.反相高效液相色谱法分离、定量及从天然胆汁中制备胆红素及其结合物。基于邻氨基苯甲酸乙酯偶氮衍生物的高效液相色谱法对完整四吡咯进行定量分析。
Biochem J. 1985 Feb 1;225(3):787-805. doi: 10.1042/bj2250787.
4
Reversed-phase high-performance liquid chromatography of conjugated and unconjugated bilirubins in body fluids.体液中共轭胆红素和非共轭胆红素的反相高效液相色谱法
J Chromatogr. 1986 Feb 26;353:19-26. doi: 10.1016/s0021-9673(01)87072-2.
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Novel method for high-performance liquid chromatography of azo derivatives of conjugated and unconjugated bilirubin.结合胆红素与非结合胆红素偶氮衍生物的高效液相色谱新方法。
J Chromatogr. 1988 May 13;427(1):19-28. doi: 10.1016/0378-4347(88)80101-4.
6
Analysis of bilirubin and bilirubin mono- and di-conjugates. Determination of their relative amounts in biological samples.胆红素及胆红素单、双结合物的分析。测定其在生物样品中的相对含量。
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A rapid and quantitative high performance liquid chromatographic method for assaying bilirubin and its conjugates in bile.一种用于测定胆汁中胆红素及其结合物的快速定量高效液相色谱法。
Can J Biochem. 1982 Nov;60(11):1050-7. doi: 10.1139/o82-135.
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High-performance liquid chromatography of bilirubin conjugates in bile: effect of beta-glucuronidase on the bile pigments.胆汁中胆红素结合物的高效液相色谱分析：β-葡萄糖醛酸酶对胆汁色素的影响
Scand J Gastroenterol. 1989 Aug;24(6):657-65. doi: 10.3109/00365528909093105.
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High-performance liquid chromatographic separation of bilirubin conjugates.胆红素结合物的高效液相色谱分离
Anal Biochem. 1989 Dec;183(2):269-74. doi: 10.1016/0003-2697(89)90478-8.

引用本文的文献

1
Investigation of the substrate specificity of a cloned expressed human bilirubin UDP-glucuronosyltransferase: UDP-sugar specificity and involvement in steroid and xenobiotic glucuronidation.克隆表达的人胆红素UDP-葡萄糖醛酸基转移酶的底物特异性研究：UDP-糖特异性及其在类固醇和外源性物质葡萄糖醛酸化中的作用。
Biochem J. 1994 Oct 1;303 ( Pt 1)(Pt 1):233-40. doi: 10.1042/bj3030233.
2
Demonstration of a geometric isomer of bilirubin-IX alpha in the serum of a hyperbilirubinaemic newborn infant and the mechanism of jaundice phototherapy.一名高胆红素血症新生儿血清中胆红素-IXα几何异构体的证实及黄疸光疗机制
Biochem J. 1980 Sep 15;190(3):533-6. doi: 10.1042/bj1900533.
3
High-pressure liquid chromatographic analysis of anaerobic photoproducts of bilirubin-IX alpha in vitro and its comparison with photoproducts in vivo.胆红素-IXα体外厌氧光产物的高压液相色谱分析及其与体内光产物的比较。
Biochem J. 1980 Sep 15;190(3):527-32. doi: 10.1042/bj1900527.
4
Bilirubin diglucuronide formation in intact rats and in isolated Gunn rat liver.完整大鼠及分离的Gunn大鼠肝脏中胆红素二葡萄糖醛酸酯的形成
J Clin Invest. 1982 Mar;69(3):595-603. doi: 10.1172/jci110486.
5
Reverse-phase h.p.l.c. separation, quantification and preparation of bilirubin and its conjugates from native bile. Quantitative analysis of the intact tetrapyrroles based on h.p.l.c. of their ethyl anthranilate azo derivatives.反相高效液相色谱法分离、定量及从天然胆汁中制备胆红素及其结合物。基于邻氨基苯甲酸乙酯偶氮衍生物的高效液相色谱法对完整四吡咯进行定量分析。
Biochem J. 1985 Feb 1;225(3):787-805. doi: 10.1042/bj2250787.
6
Application of a rapid and efficient h.p.l.c. method to measure bilirubin and its conjugates from native bile and in model bile systems. Potential use as a tool for kinetic reactions and as an aid in diagnosis of hepatobiliary disease.一种快速高效的高效液相色谱法在测定天然胆汁及模拟胆汁体系中胆红素及其结合物的应用。作为动力学反应工具及辅助肝胆疾病诊断的潜在用途。
Biochem J. 1986 Feb 15;234(1):101-9. doi: 10.1042/bj2340101.
7
Investigation of the molecular basis of the genetic deficiency of UDP-glucuronosyltransferase in Crigler-Najjar syndrome.克里格勒-纳贾尔综合征中尿苷二磷酸葡萄糖醛酸基转移酶基因缺陷的分子基础研究。
J Inherit Metab Dis. 1991;14(4):563-79. doi: 10.1007/BF01797927.

本文引用的文献

1
Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.胆红素葡萄糖醛酸苷的测定及以胆红素为受体的葡萄糖醛酸转移酶的测定。
Biochem J. 1968 Apr;107(4):507-18. doi: 10.1042/bj1070507.
2
Determination of bilirubin UDP-glucuronyl transferase activity in needle-biopsy specimens of human liver.人肝穿刺活检标本中胆红素UDP-葡萄糖醛酸基转移酶活性的测定
Clin Chim Acta. 1970 Jul;29(1):27-35. doi: 10.1016/0009-8981(70)90216-0.
3
Recent advances in the separation and analysis of diazo-positive bile pigments.重氮阳性胆汁色素分离与分析的最新进展
Methods Biochem Anal. 1974;22:205-50. doi: 10.1002/9780470110423.ch5.
4
Critique of the assay and significance of bilirubin conjugation.胆红素结合试验的评析及其意义
Adv Clin Chem. 1973;16:239-88. doi: 10.1016/s0065-2423(08)60347-9.
5
Photodegradation products of bilirubin studied by high pressure liquid chromatography, gel permeation chromatography, nuclear magnetic resonance and mass spectrometry.通过高压液相色谱、凝胶渗透色谱、核磁共振和质谱研究胆红素的光降解产物。
Birth Defects Orig Artic Ser. 1976;12(2):41-52.
6
The isolation and characterization of bilirubin diglucuronide, the major bilirubin conjugate in dog and human bile.狗和人胆汁中主要胆红素共轭物——胆红素双葡萄糖醛酸酯的分离与特性分析
Biochem J. 1976 Jun 1;155(3):477-86. doi: 10.1042/bj1550477.
7
The isolation and further characterization of the bilirubin tetrapyrroles in bile-containing human duodenal juice and dog gall-bladder bile.含胆汁的人十二指肠液和狗胆囊胆汁中胆红素四吡咯的分离及进一步特性分析
Biochem J. 1977 Oct 1;167(1):1-8. doi: 10.1042/bj1670001.
8
Conjugated and unconjugated bilirubins in humans and rhesus monkeys. Structural identity of bilirubins from biles and meconiums of newborn humans and rhesus monkeys.人类和恒河猴体内的结合胆红素与非结合胆红素。新生儿人类和恒河猴胆汁及胎粪中胆红素的结构一致性。
Biochem J. 1979 Jun 1;179(3):537-47. doi: 10.1042/bj1790537.
9
Twenty-five years of progress in bilirubin metabolism (1952-77).胆红素代谢25年的进展（1952 - 1977年）
Gut. 1978 Jun;19(6):481-91. doi: 10.1136/gut.19.6.481.