通过高压液相色谱法对各种生物流体中结合胆红素和未结合胆红素IX-α进行准确且灵敏的分析。
An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-alpha in various biological fluids.
作者信息
Onishi S, Itoh S, Kawade N, Isobe K, Sugiyama S
出版信息
Biochem J. 1980 Jan 1;185(1):281-4. doi: 10.1042/bj1850281.
Abstract
An accurate and sensitive method was developed for the complete separation of the native tetrapyrroles, such as bilirubin and its mono- and di-conjugates of glucuronic acid, glucose and xylose, by ion-pair reversed-phase high-pressure liquid chromatography. The application of this method was demonstrated by the analysis of bile pigments in human bile and urine, and the method also makes it possible to estimate very low UDP-glucuronyltransferase activity, such as is found in the human foetal and neonatal liver.
摘要
通过离子对反相高压液相色谱法，开发了一种准确且灵敏的方法，用于完全分离天然四吡咯，如胆红素及其葡萄糖醛酸、葡萄糖和木糖的单共轭物和双共轭物。通过分析人胆汁和尿液中的胆汁色素证明了该方法的应用，并且该方法还能够估计极低的尿苷二磷酸葡萄糖醛酸基转移酶活性，如在人胎儿和新生儿肝脏中发现的那样。
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An accurate and sensitive analysis by high-pressure liquid chromatography of conjugated and unconjugated bilirubin IX-alpha in various biological fluids.通过高压液相色谱法对各种生物流体中结合胆红素和未结合胆红素IX-α进行准确且灵敏的分析。
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本文引用的文献
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Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.胆红素葡萄糖醛酸苷的测定及以胆红素为受体的葡萄糖醛酸转移酶的测定。
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Determination of bilirubin UDP-glucuronyl transferase activity in needle-biopsy specimens of human liver.人肝穿刺活检标本中胆红素UDP-葡萄糖醛酸基转移酶活性的测定
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Critique of the assay and significance of bilirubin conjugation.胆红素结合试验的评析及其意义
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The isolation and characterization of bilirubin diglucuronide, the major bilirubin conjugate in dog and human bile.狗和人胆汁中主要胆红素共轭物——胆红素双葡萄糖醛酸酯的分离与特性分析
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The isolation and further characterization of the bilirubin tetrapyrroles in bile-containing human duodenal juice and dog gall-bladder bile.含胆汁的人十二指肠液和狗胆囊胆汁中胆红素四吡咯的分离及进一步特性分析
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Conjugated and unconjugated bilirubins in humans and rhesus monkeys. Structural identity of bilirubins from biles and meconiums of newborn humans and rhesus monkeys.人类和恒河猴体内的结合胆红素与非结合胆红素。新生儿人类和恒河猴胆汁及胎粪中胆红素的结构一致性。
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Twenty-five years of progress in bilirubin metabolism (1952-77).胆红素代谢25年的进展（1952 - 1977年）
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