Onishi S, Kawade N, Itoh S, Isobe K, Sugiyama S
Biochem J. 1980 Sep 15;190(3):527-32. doi: 10.1042/bj1900527.
To carry out photochemical experiments under conditions similar to those prevailing for neonatal bilirubin metabolism in jaundice phototherapy, we have studied photoproducts produced by the action of light on a bilirubin--albumin solution and further clarified the relationship between the photoproducts obtained from experiments in vitro and in vivo. (1) An accurate and sensitive separation method by high-pressure liquid chromatography for photoproducts of bilirubin under anaerobic irradiation of visible light is described. (2) There were two main photoproducts obtained from experiments both in vivo and in vitro. (3) Exact correspondence of retention time on high-pressure liquid chromatography, diazo-reactivity, thermal reversion and absorption-spectrum maxima was observed between unknown pigment and photobilirubin-IX alpha from biological fluids, and the comparable peaks 2 and 3 from experiments in vitro. (4) The behaviour of photoproducts in various solutions in the absence of light and O2 is described. (5) A lower affinity of photoproducts, especially unknown pigment, for human serum albumin than with bilirubin-IX alpha for the albumin was demonstrated by the gel-filtration method.
为了在类似于黄疸光疗中新生儿胆红素代谢的条件下进行光化学实验，我们研究了光对胆红素 - 白蛋白溶液作用产生的光产物，并进一步阐明了体外和体内实验获得的光产物之间的关系。（1）描述了一种通过高压液相色谱法对胆红素在可见光厌氧照射下的光产物进行准确、灵敏分离的方法。（2）体内和体外实验均得到两种主要光产物。（3）在生物流体中的未知色素与光胆红素 - IXα之间，以及体外实验中可比的峰2和峰3之间，观察到高压液相色谱保留时间、重氮反应性、热逆转和吸收光谱最大值的精确对应。（4）描述了在无光和无氧条件下光产物在各种溶液中的行为。（5）通过凝胶过滤法证明，光产物，尤其是未知色素，与人血清白蛋白的亲和力低于胆红素 - IXα与白蛋白的亲和力。
